Protein Info for AO353_02025 in Pseudomonas fluorescens FW300-N2E3

Annotation: mannose-1-phosphate guanyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase" amino acids 3 to 477 (475 residues), 590.8 bits, see alignment E=1e-181 PF00483: NTP_transferase" amino acids 5 to 291 (287 residues), 173.1 bits, see alignment E=2e-54 PF12804: NTP_transf_3" amino acids 6 to 135 (130 residues), 28.2 bits, see alignment E=5.1e-10 PF22640: ManC_GMP_beta-helix" amino acids 303 to 355 (53 residues), 58.7 bits, see alignment 1.1e-19 PF01050: MannoseP_isomer" amino acids 361 to 473 (113 residues), 172.7 bits, see alignment E=7.2e-55 PF07883: Cupin_2" amino acids 390 to 456 (67 residues), 32 bits, see alignment E=2.1e-11

Best Hits

Swiss-Prot: 47% identical to ALGA_PSEFL: Alginate biosynthesis protein AlgA (algA) from Pseudomonas fluorescens

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] (inferred from 80% identity to ppw:PputW619_1383)

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22

Use Curated BLAST to search for 2.7.7.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WE21 at UniProt or InterPro

Protein Sequence (486 amino acids)

>AO353_02025 mannose-1-phosphate guanyltransferase (Pseudomonas fluorescens FW300-N2E3)
MELIPVILSGGVGSRLWPVSREAHPKPFMDLPGGQNLIQKTFLRASQLEGVVEVLTVTNR
ELLFKTEDEYGAVNTAKHSQGFILEPFGRNTAAAVAAAALQLEATHGSNVHMLVLAADHL
IKDEKAFAAAVANAVTLAAEGWLVTFGIQPTYPETGFGYIEAQKNAPLNGGLKVARFVEK
PDLETAQGYVGAGNYYWNSGMFCFRVGTVLEELREHAPDVVEAVARTIEQSRVTSSDKYR
CLALDAEAFAEVPDISIDYALMERSAKVATIPCDIGWSDIGSWNAVAELTAPDADGNRFE
GEVLSHGSRNNYVNSEGRLTALVGVEDLLVIDTPDAVMIAHKDHAQDVKHIVGQLKANSH
TVHLLHRTVHRPWGTYTTLENGERFKIKRIVVKPKASLSLQMHHHRSEHWIVVSGMAVVI
NDQQELMLNTNESTFIRAGHKHRLQNPGVIDLVMIEVQSGDYLGEDDIVRFEDNYGRVDG
VATTKF