Protein Info for AO353_01970 in Pseudomonas fluorescens FW300-N2E3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 84 to 106 (23 residues), see Phobius details amino acids 118 to 135 (18 residues), see Phobius details PF13727: CoA_binding_3" amino acids 70 to 255 (186 residues), 29.8 bits, see alignment E=1.8e-10 PF04321: RmlD_sub_bind" amino acids 299 to 429 (131 residues), 30.5 bits, see alignment E=6e-11 PF01370: Epimerase" amino acids 300 to 532 (233 residues), 53.6 bits, see alignment E=6.7e-18 PF02719: Polysacc_synt_2" amino acids 300 to 607 (308 residues), 409.8 bits, see alignment E=1.9e-126 PF16363: GDP_Man_Dehyd" amino acids 301 to 428 (128 residues), 38.2 bits, see alignment E=3.6e-13 PF01073: 3Beta_HSD" amino acids 301 to 433 (133 residues), 28.8 bits, see alignment E=1.9e-10

Best Hits

KEGG orthology group: None (inferred from 90% identity to pfo:Pfl01_4051)

MetaCyc: 76% identical to UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (Pseudomonas aeruginosa)
RXN-13252 [EC: 4.2.1.135]

Predicted SEED Role

"nucleotide sugar epimerase/dehydratase WbpM"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WE09 at UniProt or InterPro

Protein Sequence (664 amino acids)

>AO353_01970 hypothetical protein (Pseudomonas fluorescens FW300-N2E3)
MDKVRAFLLGLPRRKKRLIQVITDMVLVWVALWLAFIVRLGIDEMATPLKIHLWLFACAP
VIAIPLFIRFGMYRAVMRYFGNDALIAIVKAVSLSSLILAVIVYWYSNHQVVVPRSIIFN
YWWLSLVIIGGLRLCMRQYFMGDWFTAAQHVPFTNRDDGLTKVAIYGAGVAGNQLVAALR
MGRVMRPVAFIDDDASIADRSISGLQVYKPKHIQQMIDVTGAQEILLALPSSTRARRREI
LNLLEGFPLHIRSVPNFTDLASGRVKVEDIQEVDIADLLGRDSVPAQPDLLERCIKRKTV
MVTGAGGSIGAELCRQIFSLGPTTLILFEHSEFNLYSILSELEKRASRESLSVRLLPILG
SVRHQDKLLDVMKTWKVETVYHAAAYKHVPMVEHNIAEGVLNNVMGTLNTAQAALQSGVA
NFVLISTDKAVRPTNVMGSTKRLAEMTLQALSREIAPVLLGDKANVSRVNKTRFTMVRFG
NVLGSSGSVIPLFHSQIKSGGPLTVTHPKITRYFMTIPEAAQLVIQAGSMGQGGDVFVLD
MGEPVKIVELAEKMIHLSGLSIRSEKNLQGDIAIEFTGLRPGEKLYEELLIGDNVAATQH
PMIMSANEDHLPWDVLKTRLTELLGAVERDDYSRVRQLLRETVSGYTPDGEIVDWIYQQR
RLEP