Protein Info for AO353_01955 in Pseudomonas fluorescens FW300-N2E3

Annotation: thiosulfate transporter subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 20 to 328 (309 residues), 426.8 bits, see alignment E=2.6e-132 PF13531: SBP_bac_11" amino acids 31 to 279 (249 residues), 125.5 bits, see alignment E=5.9e-40 PF01547: SBP_bac_1" amino acids 41 to 274 (234 residues), 49.2 bits, see alignment E=1.6e-16 PF13416: SBP_bac_8" amino acids 85 to 285 (201 residues), 29.3 bits, see alignment E=1.5e-10 PF13343: SBP_bac_6" amino acids 132 to 289 (158 residues), 25.5 bits, see alignment E=1.7e-09

Best Hits

Swiss-Prot: 54% identical to SUBI_SALTY: Sulfate-binding protein (sbp) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 94% identity to pfl:PFL_1694)

MetaCyc: 54% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPJ5 at UniProt or InterPro

Protein Sequence (332 amino acids)

>AO353_01955 thiosulfate transporter subunit (Pseudomonas fluorescens FW300-N2E3)
VKKIFGASLLAAGLTLASVAQAAPTLLNVSYDVMRDFYKDYNTAFQKHWQAEHNENITLQ
MSFGGSSKQARSVIDGLPADVITMNMATDINALVDNGKLVPDNWVTRLPDHSAPFTSATV
FIVRKGNPKALKDWPDLLKDGVQVIVPNPKTSGNGRYTYLSAWGYVLKNGGDENKAKAFV
GKLFKQAPVLDTGGRAATTTFMTNQIGDVLVTFENEAEMIAREFGRDQFEVIYPSVSAEA
EPPVSVVDKVVDKKGTRAAAEEYLKYLWSPEGQEIAAANYLRPRDPAVLAKHTDRFPKVD
FLSVEKTFGDWRTVQKTHFNDGGVFDQIYSGQ