Protein Info for AO353_01920 in Pseudomonas fluorescens FW300-N2E3

Annotation: glyoxylate carboligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 TIGR01504: glyoxylate carboligase" amino acids 3 to 590 (588 residues), 1167.8 bits, see alignment E=0 PF02776: TPP_enzyme_N" amino acids 4 to 121 (118 residues), 110.7 bits, see alignment E=6e-36 PF00205: TPP_enzyme_M" amino acids 193 to 327 (135 residues), 130.6 bits, see alignment E=4.9e-42 PF02775: TPP_enzyme_C" amino acids 393 to 553 (161 residues), 117.7 bits, see alignment E=6.1e-38

Best Hits

Swiss-Prot: 74% identical to GCL_ECOLI: Glyoxylate carboligase (gcl) from Escherichia coli (strain K12)

KEGG orthology group: K01608, tartronate-semialdehyde synthase [EC: 4.1.1.47] (inferred from 97% identity to pfo:Pfl01_1598)

MetaCyc: 74% identical to glyoxylate carboligase (Escherichia coli K-12 substr. MG1655)
Tartronate-semialdehyde synthase. [EC: 4.1.1.47]

Predicted SEED Role

"Glyoxylate carboligase (EC 4.1.1.47)" in subsystem Allantoin Utilization or Photorespiration (oxidative C2 cycle) (EC 4.1.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZB3 at UniProt or InterPro

Protein Sequence (591 amino acids)

>AO353_01920 glyoxylate carboligase (Pseudomonas fluorescens FW300-N2E3)
MSKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEG
YTRTKAGNIGVCIGTSGPAGTDMVTGLYSASADSIPILCITGQAPRARMHKEDFQAVDIT
SIVKPVTKWATTVLEPGQVPYAFQKAFYEMRSGRPGPVLIDLPFDVQMAEIEFDIDTYEP
LPLAKPAATRKQVEKALAMLDQAERPLLVAGGGIINADASDLLVEFAELTGIPVIPTLMG
WGTIPDDHPLMVGMVGLQTSHRYGNATMLKSDVVLGVGNRWANRHTGSVDVYTEGRKFIH
VDIEPTQIGRVFTPDQGIVSDAGLALKMFIEVAREWKVAGKLKDRSAWLQDCQQRKASLH
RKTHFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAGAQFLHVYKPRHWINCGQAGP
LGWTIPAALGVVKADPSRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIR
QAQRGFDMDYCVQLSFDNLNAPELNGYGVDHVAVAEGLGCKALRVFEPAGIAPALRKAEE
LIEEFKVPVIVEIILERVTNISMGTEINAVNEFEDLALVGSDAPTAISLLD