Protein Info for AO353_01780 in Pseudomonas fluorescens FW300-N2E3

Annotation: heat-shock protein Hsp90

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 PF02518: HATPase_c" amino acids 32 to 185 (154 residues), 29.9 bits, see alignment E=1e-10 PF13589: HATPase_c_3" amino acids 32 to 157 (126 residues), 35.5 bits, see alignment E=1.5e-12 PF00183: HSP90" amino acids 224 to 629 (406 residues), 431 bits, see alignment E=1.2e-132

Best Hits

Swiss-Prot: 95% identical to HTPG_PSEF5: Chaperone protein HtpG (htpG) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 96% identity to pba:PSEBR_a4129)

MetaCyc: 60% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X2L9 at UniProt or InterPro

Protein Sequence (634 amino acids)

>AO353_01780 heat-shock protein Hsp90 (Pseudomonas fluorescens FW300-N2E3)
MSVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAVDKLRFEALSKPEWL
EGGAELKIRVSFDKDAKTVTLEDNGIGMSRDDVVTHLGTIAKSGTADFMKHLSGDQKKDS
HLIGQFGVGFYSAFIVADKVDVYSRRAGLAASEGVHWSSKGEGEFDVATIDKEDRGTRIV
LHLKSAEDEFADGWRLRNIIKKYSDHIALPIELPKEVAAAEGEEKPAVEWETVNRASALW
TRPRTEVKDEEYQEFYKHIAHDFENPLSWSHNKVEGKLEYNSLLYVPARAPFDLYQREAP
RGLKLYVQRVFVMDQAESFLPLYLRFIKGVVDSNDLSLNVSREILQKDPIIDSMKSALTK
RVLDMLEKLAKNEPEQYKGFWKNFGQVMKEGPAEDFANKEKIAGLLRFASTQGEEGEQVV
SLTEYLARAKEGQDKIYYLTGETYAQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYLSD
FDGKSFVDVARGDLDLGNLDSAEDKKAAEEVAKSKEGLVERIKTALGETVSEVRVSHRLT
DSPAILAIGEQDLGLQMRQILEASGQKVPDSKPIFEFNPAHPLIEKLDGEQSEERFGDLS
HILFDQAALAAGDSLKDPAAYVRRLNKLLVELSV