Protein Info for AO353_01420 in Pseudomonas fluorescens FW300-N2E3

Annotation: acylaldehyde oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 771 transmembrane" amino acids 19 to 36 (18 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 13 to 35 (23 residues), 21.3 bits, see alignment (E = 1.2e-08) PF20256: MoCoBD_2" amino acids 59 to 203 (145 residues), 56.2 bits, see alignment E=4.1e-19 amino acids 627 to 708 (82 residues), 57.4 bits, see alignment E=1.7e-19 PF02738: MoCoBD_1" amino acids 331 to 563 (233 residues), 115 bits, see alignment E=3.2e-37

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 90% identity to pfl:PFL_1784)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WDT8 at UniProt or InterPro

Protein Sequence (771 amino acids)

>AO353_01420 acylaldehyde oxidase (Pseudomonas fluorescens FW300-N2E3)
MSQLPNDFALSNLSRRGFLKGVSATGALVLAASWGWQDAFAEEKKFGAAGMPNGWIDDPK
VYVSIAADGSVTVVCNRSEMGQGVRTSLSMVVADELDADWAMVKVRQAPGDEVRFGNQDT
DGSRSMRHWYEPMRRCGAAARTMLEQAAADQWKVPVGECRAQLHKVIHQPTGRELGYGAL
AVAAGALAVPARESLRLKQSSEFRYIGKEGTKAIDGADIVNGRAVYGADVHFDGMLFATV
ARPAVYGGKVKSFDASAALKVPGVVKVIQIESRPLPSEFQPLGGVAVIASNTWAAIKGRE
ALKIEWDDGANAGYDSIVYRKELEAAALKPGKVVRSTGNIEEAMNSADSSLEAAYYLPHL
AQSPMEPMVAVARFKDGHCEAWAPSQAPQVTRERIAERLGIPFDKVTVNVTLLGGGFGRK
SKPDFILEAAILAKEFPGKAVRVQWTREDDIHCSYFHTVSAEYLKASLNKDGMPSGWLHR
TVAPSITALFAPGMNHEAAFELGMGFTNMAYAIPNVRLENPDAAAHTRVGWYRSVSNIPH
GFAIQSFVDELAHKAGQDPLKYQIKLLGPDRQIDPRTLSEEWNYGESPERYPIDTARLRG
VLETAAKGAGWGRKLPKGRGLGLAVHYSFVTYVAAVIEVEVKDDGTLIVHKADIAVDCGP
QINPERIRSQFEGACVMGLGNAVLGEISFKDGKVQQDNFHMYEVARMSLAPKEVAVHLVT
PAGNVPLGGVGEPGVPPIAPALCNAIFAATGKRIRSLPVRYQLQGWQQSKA