Protein Info for AO353_01375 in Pseudomonas fluorescens FW300-N2E3

Annotation: phosphate acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02504: FA_synthesis
  • amino acids 1 to 315 (315 residues), 319.7 bits, E=2e-99
• TIGR00182: fatty acid/phospholipid synthesis protein PlsX
  • amino acids 1 to 323 (323 residues), 328.5 bits, E=2.3e-102
• PF01515: PTA_PTB
  • amino acids 76 to 205 (130 residues), 26.1 bits, E=5.5e-10

Best Hits

Swiss-Prot: 94% identical to PLSX_PSEPF: Phosphate acyltransferase (plsX) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03621, glycerol-3-phosphate acyltransferase PlsX [EC: 2.3.1.15] (inferred from 94% identity to pfl:PFL_1794)

Predicted SEED Role

"Phosphate:acyl-ACP acyltransferase PlsX" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria

MetaCyc Pathways

• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (9/13 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (3/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Sequence Analysis Tools

See A0A0N9W6B1 at UniProt or InterPro

Protein Sequence (326 amino acids)

>AO353_01375 phosphate acyltransferase (Pseudomonas fluorescens FW300-N2E3)
MGGDFGPRSIVQASLACLSATPSLHLTLVGQPSLLEELIASQSAVDRARLTIVPASDVIG
MDEKPSQALRGKPDSSMRVALELLRDGKVQACVSAGNTGALMALSRYVLKTLPGIDRPAM
VAAIPTQRGYCQLLDLGANVDCSAEHLFQFAVMGSVAAETLGIARPKVALLNIGTEDIKG
NQQVKLAATLLQNARGLNYIGFVEGDGLYRGEADVVVCDGFVGNILLKSSEGLATMIAGR
IEALFKQNLASRMVGALALPLMKRLQADLAPARHNGASFLGLQGIVVKSHGSAGVQGFQS
AIQRALIEIQENLPQRLHGRLEDLLL