Protein Info for AO353_00970 in Pseudomonas fluorescens FW300-N2E3

Annotation: group II intron reverse transcriptase/maturase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 TIGR04416: group II intron reverse transcriptase/maturase" amino acids 63 to 406 (344 residues), 409.5 bits, see alignment E=6.2e-127 PF00078: RVT_1" amino acids 119 to 324 (206 residues), 140.5 bits, see alignment E=6e-45 PF08388: GIIM" amino acids 342 to 419 (78 residues), 72.5 bits, see alignment E=2.3e-24

Best Hits

KEGG orthology group: K00986, RNA-directed DNA polymerase [EC: 2.7.7.49] (inferred from 90% identity to ajs:Ajs_2219)

Predicted SEED Role

"Retron-type RNA-directed DNA polymerase (EC 2.7.7.49)" (EC 2.7.7.49)

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.49

Use Curated BLAST to search for 2.7.7.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WNG3 at UniProt or InterPro

Protein Sequence (473 amino acids)

>AO353_00970 group II intron reverse transcriptase/maturase (Pseudomonas fluorescens FW300-N2E3)
MPPVGVRVSLNTEMQKFLPEKTVTPNPGQNPRMTADSAQVSAASVTWTNAESDTLMARVL
APANLRCAYQRVVSNKGAPGADGMTVDDLASYVKQYWPILKARLLAGEYHPQGVRAVDIP
KPKGGTRQLGIPGVVDRLIQQALLQQLTPIFDPLFSDYSYGFRPGRSAHQAIETARAHVA
AGHRWCVELDLEKLFDRVNHDVLMAYVQRHVEDRRVLTLIRRYLQAGVMSGGIASRRQEG
TPQGGPLSPLLSNILLNELDRELERRGHRFVRYADDANIYVRSRRAGERVMVSVERFLSQ
RLKLTLNREKSRVARPWVCDYLGYGMSWHQQPKLRVASMSLHRLRDRLRELLRRIRSRSM
RYIIEHITPVLRGWAGYFKLSQSKRPLEELDGWVRHKLRCVTWRQWKRPSTRARNLMRLG
LDEARAWKSAFNGRGPWWNSGAPHMNQALPKKLWDRLGLVSILDTINRLSRIT