Protein Info for AO353_00895 in Pseudomonas fluorescens FW300-N2E3

Annotation: 2-methylcitrate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 PF00285: Citrate_synt" amino acids 11 to 358 (348 residues), 430.1 bits, see alignment E=3.5e-133 TIGR01800: 2-methylcitrate synthase/citrate synthase II" amino acids 11 to 375 (365 residues), 512.7 bits, see alignment E=2.3e-158

Best Hits

Swiss-Prot: 74% identical to PRPC_SHEON: 2-methylcitrate synthase (prpC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01659, 2-methylcitrate synthase [EC: 2.3.3.5] (inferred from 94% identity to pfo:Pfl01_1765)

MetaCyc: 52% identical to 2-methylcitrate synthase (Cupriavidus necator)
2-methylcitrate synthase. [EC: 2.3.3.5]

Predicted SEED Role

"2-methylcitrate synthase (EC 2.3.3.5)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 2.3.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VZZ6 at UniProt or InterPro

Protein Sequence (375 amino acids)

>AO353_00895 2-methylcitrate synthase (Pseudomonas fluorescens FW300-N2E3)
MAEAKVLSGAGLRGQVAGQTALSTVGQEGAGLTYRGYDVRELAAQAHFEEVAYMLLYGEL
PNKAQLAAYINKLSKLRDLPQALKEVLERIPADAHPMDVMRTGCSFLGNLEPEHDFSEQH
DKTDRLLAAFPAIMCYWYRFSHDGKRINCVTDEESIGGHFLHLLHDKKPSELHVKVMNVS
LILYAEHEFNASTFTARVCASTLSDLFSCVTAAIGSLRGPLHGGANEAAMEMIERFASPQ
EAIKGTLDMLARKDKIMGFGHAIYKDNDPRNEVIKGWSKKLSDEVGDTVLFPVSEAIDKT
MWEQKKLFPNADFYHASAYHFMGIPTKLFTPIFVCSRLTGWAAHVFEQRANNRIIRPSAE
YVGVEQRKFVPIEQR