Protein Info for AO353_00815 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Nitrate reductase (EC 1.7.99.4)
Rationale: Specifically important for utilizing Sodium nitrate and Sodium nitrite. Automated validation from mutant phenotype: the predicted function (1.7.99.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 905 PF04879: Molybdop_Fe4S4" amino acids 4 to 52 (49 residues), 60.7 bits, see alignment 2.2e-20 PF00384: Molybdopterin" amino acids 63 to 488 (426 residues), 242.2 bits, see alignment E=1.8e-75 PF01568: Molydop_binding" amino acids 587 to 695 (109 residues), 88.3 bits, see alignment E=7.2e-29 PF04324: Fer2_BFD" amino acids 849 to 895 (47 residues), 31.7 bits, see alignment 3.1e-11

Best Hits

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 90% identity to pfo:Pfl01_1779)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WDK0 at UniProt or InterPro

Protein Sequence (905 amino acids)

>AO353_00815 Nitrate reductase (EC 1.7.99.4) (Pseudomonas fluorescens FW300-N2E3)
MSRQTTASTCCYCGVGCGVLIEHDGEQILGVSGDPTHPANFGKLCSKGSTLHLTGDLAAR
ALYPELRLGKGMARSRTDWDTALDHATSVFADTIAEHGPDSVAFYISGQLLTEDYYAFNK
LARALVGTNNIDSNSRLCMSSAVVGYKRSLGADAPPCSYEDLELSDCVMIVGSNMAYAHP
VLFRRLEEAKSRRPQMKVIVIDPRRTDTCDLADLHLAILPGTDVALFHGILHLLLWEDWV
DREFIKAHTEGLAELKSLVRDYTPQMVAQLCGISVEQLQQCAEWVGTSSSFLSLWCMGLN
QSTAGSAKNSALINLHLATGQIGRPGTGPFSLTGQPNAMGGRETGSLSNLLPGHREAANA
QHRAEVAAYWGVEKLPDTTGLTAIELFEQVQSGKIKALWIACTNPAQSLPDQQTVRAALQ
ACPFVVLQEAFRTTETAPFADLLLPAASWGEKEGTVTNSERRISHVRRAINAPGEARPDW
AITVDFAQRLERRLRPGQPSLFAFEQPAQVFDEYKQLTRGRDLDLCGISHALLDRLGPQQ
WPFPDGASSGTPRLYADGIFPTESGRAQFVADPYRAAKEQRDARFPLTLITGRLRDQWHG
MSRTGTAAQLFGHVSEALLSLHPDELRRYRLQTGDLVNLKSRRGSVIVAIGSDDSVRPGQ
AFLPMHWGDRFLKGGVNTLTQPAFDPLSKQPELKHSGVRIEPVKLPWHLFALVEGDVQQH
FETLRPLCEDFSYASLSLTGRERPALLIRAASAQAPDPQRLHAIDQQLGLNDGPVLAYDD
PRRAIGKRVRIEQGRITAIRLAGETLAQHWLQNLWLEGRADEQLRRWLLAPLSTPPGSAG
SSVSTTKTLCNCKNVSESAVCAGIRSGLDLQGLKQQLGCGTQCGSCVPEIKRLLAANVQP
IAITS