Protein Info for AO353_00805 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Nitrite reductase (NAD(P)H) (EC 1.7.1.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 817 PF07992: Pyr_redox_2" amino acids 5 to 283 (279 residues), 204 bits, see alignment E=1.1e-63 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 799 (794 residues), 928 bits, see alignment E=2.9e-283 PF00070: Pyr_redox" amino acids 147 to 228 (82 residues), 58.3 bits, see alignment E=2.7e-19 PF18267: Rubredoxin_C" amino acids 319 to 385 (67 residues), 60 bits, see alignment E=5.4e-20 PF04324: Fer2_BFD" amino acids 423 to 471 (49 residues), 52.2 bits, see alignment 1.9e-17 amino acids 488 to 538 (51 residues), 47 bits, see alignment 8.1e-16 PF03460: NIR_SIR_ferr" amino acids 563 to 625 (63 residues), 54.5 bits, see alignment 2.4e-18 PF01077: NIR_SIR" amino acids 633 to 770 (138 residues), 115.3 bits, see alignment E=5.8e-37

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 80% identity to avn:Avin_23370)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WP46 at UniProt or InterPro

Protein Sequence (817 amino acids)

>AO353_00805 Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (Pseudomonas fluorescens FW300-N2E3)
MKKLKLVMIGNGMAGVRTLEELLKLSSELYDITVFGAEPHTNYNRILLSPVLAGEQTFEE
IVLNDLDWYLENNIKLLLNRKVVEIDRVKRRVIAEDGSEAEYDRLLIATGSTPFILPIPG
NTLEGVIGYRDIADTQVMIDTAKTHKHAVVIGGGLLGLEAANGLMLRGMHVTVVHLGEWL
LERQLDKTSGQLLQTALEGRGLHFRLCEQTQALHDAGNGRVGSVQFKNGDIIPADLVVMA
AGIRPNTELAEKSGIPCNRGILVNDTLQTYDPRIYAIGECASHRGIAYGLVAPLFEQAKV
CANHLAQLGFARYSGSVTSTKLKVTGIDLFSAGDFMGGEGTETITLSDPIGGVYKKLVIK
DDVLVGACLYGDTADGGWYFRQIRENHAIGEIRDHLMFGENALGDVGHQGQDKAMSMADN
AEVCGCNGVCKGTIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILINTVGGAADV
KPKSEKAICGCSDLNHGQIRQAIREEHLLTIAGTMSYLNWRTPNGCATCRPALNYYLIST
WPGEAKDDPQSRLINERAHANIQKDGTYSVVPRMWGGVTNPSELRRIADVADKYNVPMVK
VTGGQRIDLLGIKKQDLPGVWKDLDMPSGHAYGKSIRTVKTCVGSEFCRFGTQNSTQLGI
ELEHDLFNMWSPHKVKLAVSGCPRNCSEAGIKDVGIIGVDSGWEMYIGGNGGIKTEVAEF
FVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIKKAVLEDPERRKALNDR
LQFSLSFEQDPWKERLEQPLLKKEFDVIPVKNLEVPA