Protein Info for AO353_00795 in Pseudomonas fluorescens FW300-N2E3

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 51 to 71 (21 residues), see Phobius details amino acids 79 to 99 (21 residues), see Phobius details amino acids 105 to 126 (22 residues), see Phobius details amino acids 138 to 161 (24 residues), see Phobius details amino acids 168 to 187 (20 residues), see Phobius details amino acids 212 to 235 (24 residues), see Phobius details amino acids 247 to 265 (19 residues), see Phobius details amino acids 276 to 294 (19 residues), see Phobius details amino acids 300 to 321 (22 residues), see Phobius details amino acids 332 to 356 (25 residues), see Phobius details amino acids 363 to 382 (20 residues), see Phobius details TIGR00886: nitrite transporter" amino acids 13 to 347 (335 residues), 313.4 bits, see alignment E=1.1e-97 PF07690: MFS_1" amino acids 23 to 349 (327 residues), 159.4 bits, see alignment E=1.2e-50 PF13347: MFS_2" amino acids 149 to 319 (171 residues), 28.9 bits, see alignment E=4.3e-11

Best Hits

Swiss-Prot: 48% identical to NASA_BACSU: Nitrate transporter (nasA) from Bacillus subtilis (strain 168)

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 96% identity to pfo:Pfl01_1783)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WCJ1 at UniProt or InterPro

Protein Sequence (403 amino acids)

>AO353_00795 MFS transporter (Pseudomonas fluorescens FW300-N2E3)
MNSSFWKSGHTPTLFSAFLYFDLSFMVWYLLGPLAVQIAADLHLTTQQRGLVVATPILAG
AFLRFVMGLLADRLSPKTAGLIGQVIVICALFGAWKLGIHSYEQALLLGLFLGMAGASFA
VALPLASQWYPPQHQGKAMGIAGAGNSGTVFAALIAPMLAASFGWSNVFGFALIPLILTL
IIFAWLAKNAPERPKAKSMTDYFKALGDRDSWWFMFFYSVTFGGFIGLASALPGYFNDQY
GLSPVTAGYYTAACVFGGSLMRPLGGALADRFGGIRTLLVMYTLAATCIAAVGFNLPSSY
AALALFVCTMLGLGAGNGAVFQLVPQRFRREIGVMTGLIGMAGGIGGFALAAGMGAIKQS
TGSYQLALWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTAARV