Protein Info for AO353_00785 in Pseudomonas fluorescens FW300-N2E3

Annotation: nitrate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 80 to 103 (24 residues), see Phobius details PF13379: NMT1_2" amino acids 16 to 267 (252 residues), 319 bits, see alignment E=1.4e-99

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 89% identity to pfo:Pfl01_1785)

Predicted SEED Role

"Nitrate ABC transporter, nitrate-binding protein" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WJJ7 at UniProt or InterPro

Protein Sequence (403 amino acids)

>AO353_00785 nitrate transporter (Pseudomonas fluorescens FW300-N2E3)
MTEPEAGPLAWVNGSDAPEKTAINLGFMALSDCASVVVAATQGFAQPYGLTLNLKRQSSW
ANLRDKLVSGELDAAHSLYGLIYAVHLGIGGVAATDMAVLMGLNQNGQSINLSHGLQALG
VTGPEALDRHVHQSRPKLTFAQTFPTGTHAMWLYYWLASQGIHPLQDVDSVVVPPPQMVA
HLQAGRIDGFCVGEPWSASAVKQNLGFTLATSQTIWPDHPEKVLGCTRAFIEQYPNTARA
LVMAVLEASRFIEQSTENRRSTAQLLSAAEYLDAPLDCIEPRLLGDYADGLGNRWQDPHA
LRFHGGGDVNLPYLSDGMWFMTQFRRWGLLREDPDYLQVARQVQQLGVYRDAASALGIAA
RDQEMRSSQLIDGKIWNGSDPAAYARSFKLHAMADTPALLARR