Protein Info for AO353_00510 in Pseudomonas fluorescens FW300-N2E3

Annotation: multifunctional fatty acid oxidation complex subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 97% identical to FADB_PSEPF: Fatty acid oxidation complex subunit alpha (fadB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01825, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase / enoyl-CoA isomerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3 5.3.3.8] (inferred from 97% identity to pfo:Pfl01_3879)

MetaCyc: 90% identical to FadB (Pseudomonas putida KT2440)
Dodecenoyl-CoA isomerase. [EC: 5.3.3.8]; 3-hydroxybutyryl-CoA epimerase. [EC: 5.3.3.8, 5.1.2.3]; Enoyl-CoA hydratase. [EC: 5.3.3.8, 5.1.2.3, 4.2.1.17]; Acetoacetyl-CoA reductase. [EC: 5.3.3.8, 5.1.2.3, 4.2.1.17, 1.1.1.36]; 3-hydroxyacyl-CoA dehydrogenase. [EC: 5.3.3.8, 5.1.2.3, 4.2.1.17, 1.1.1.36, 1.1.1.35]

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

oleate β-oxidation (30/35 steps found)
fatty acid salvage (6/6 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (5/5 steps found)
adipate degradation (5/5 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
2-methyl-branched fatty acid β-oxidation (11/14 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
polyhydroxybutanoate biosynthesis (3/3 steps found)
L-isoleucine degradation I (5/6 steps found)
oleate β-oxidation (thioesterase-dependent, yeast) (2/2 steps found)
valproate β-oxidation (7/9 steps found)
adipate biosynthesis (4/5 steps found)
fatty acid β-oxidation IV (unsaturated, even number) (4/5 steps found)
fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
L-valine degradation I (6/8 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
propanoate fermentation to 2-methylbutanoate (4/6 steps found)
pyruvate fermentation to butanol II (engineered) (4/6 steps found)
1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
4-hydroxybenzoate biosynthesis III (plants) (3/5 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
(2S)-ethylmalonyl-CoA biosynthesis (2/4 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
acetyl-CoA fermentation to butanoate (4/7 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
methyl ketone biosynthesis (engineered) (3/6 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
glycerol degradation to butanol (10/16 steps found)
pyruvate fermentation to butanol I (4/8 steps found)
oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
benzoyl-CoA degradation I (aerobic) (3/7 steps found)
pyruvate fermentation to butanoate (3/7 steps found)
6-gingerol analog biosynthesis (engineered) (2/6 steps found)
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
3-phenylpropanoate degradation (4/10 steps found)
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
phenylacetate degradation I (aerobic) (3/9 steps found)
2-methylpropene degradation (2/8 steps found)
superpathway of phenylethylamine degradation (4/11 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
4-oxopentanoate degradation (2/9 steps found)
L-tryptophan degradation III (eukaryotic) (6/15 steps found)
gallate degradation III (anaerobic) (3/11 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
methyl tert-butyl ether degradation (2/10 steps found)
L-glutamate degradation VII (to butanoate) (3/12 steps found)
ethylmalonyl-CoA pathway (2/11 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (3/13 steps found)
10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (3/14 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
toluene degradation VI (anaerobic) (3/18 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
androstenedione degradation I (aerobic) (7/25 steps found)
Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
platensimycin biosynthesis (6/26 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
superpathway of testosterone and androsterone degradation (7/28 steps found)
androstenedione degradation II (anaerobic) (5/27 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (9/42 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (10/47 steps found)
superpathway of cholesterol degradation III (oxidase) (6/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 1.1.1.36, 4.2.1.17, 5.1.2.3

Use Curated BLAST to search for 1.1.1.35 or 1.1.1.36 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VNT6 at UniProt or InterPro

Protein Sequence (715 amino acids)

>AO353_00510 multifunctional fatty acid oxidation complex subunit alpha (Pseudomonas fluorescens FW300-N2E3)
MIYEGKAITVKALESGIVELKFDLKGESVNKFNRLTLNELRSAVDAIKADASVKGVIVSS
GKDVFIVGADITEFVDNFKLPDAELVAGNLEANKIFSDFEDLNVPTVAAINGIALGGGLE
MCLAADYRVMSTTAKIGLPEVKLGIYPGFGGTVRLPRIIGADNAIEWIAAGKENRPEDAL
KVGAVDAVVAPEQLQEAALELLKRAISGEFDYKAKRQPKLEKLKLNAIEQMMAFETAKGF
VAGQAGPNYPAPVEAIKTIQKAANFGRDKALEVEANGFVKLAKTSAAQSLIGLFLNDQEL
KKKAKAYDEIAKDVKQAAVLGAGIMGGGIAYQSASKGTPILMKDINEHGIEQGLAEAAKL
LVGRVDKGRMTAAKMAEVLNGIRPTLSYGDFGHVDLVVEAVVENPNVKQAVLAEVEDKVK
EGTILASNTSTISISLLAKALKRPENFVGMHFFNPVHMMPLVEVIRGEKSSEQAIATTVA
YAKKMGKNPIVVNDCPGFLVNRVLFPYFGGFAKLVSAGVDFVRIDKVMEKFGWPMGPAYL
MDVVGIDTGHHGRDVMAEGFPDRMKDDRRSAVDVLYEAKRLGQKNGKGFYAYETDKRGKQ
KKVADPSVLEVLKPIVFEQREVTDEDIINWMMIPLCLETVRCLEDGIVETAAEADMGLVY
GIGFPPFRGGALRYIDAIGVAEFVALADQYADLGALYHPTAKLREMAKNGQSFFG