Protein Info for AO353_00360 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 157 to 177 (21 residues), see Phobius details PF21085: CusS" amino acids 2 to 151 (150 residues), 35.5 bits, see alignment E=2.8e-12 TIGR01386: heavy metal sensor kinase" amino acids 3 to 450 (448 residues), 514.3 bits, see alignment E=1.6e-158 PF00672: HAMP" amino acids 175 to 227 (53 residues), 40.3 bits, see alignment 7.8e-14 PF00512: HisKA" amino acids 233 to 298 (66 residues), 57.6 bits, see alignment E=2.6e-19 PF02518: HATPase_c" amino acids 341 to 451 (111 residues), 90.3 bits, see alignment E=2.8e-29

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 79% identity to pfl:PFL_1964)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZ76 at UniProt or InterPro

Protein Sequence (453 amino acids)

>AO353_00360 ATPase (Pseudomonas fluorescens FW300-N2E3)
MRRLSLSGRLALLFAACTAVVSLFAGVLFSRASETHFIELDQQLLDGKLIALRSVLAGAD
DPRLFAERKAGLQDEMSHQPDLALRVRGADGHVWFDSAANLPDDLPTRSGLHSLHSADTA
YRVYSAALEPSKTDSAQLILMLDITHHQHFLQRMQHLIWLTVGLSALATALLGAWAARSG
LRPLRRMSEVAAGVSASSLTQRLPEDQMPAELANLAHTFNAMLGRLDDSFQRLSAFSADI
AHELRTPLSNLLTHTQVTLTRPRDLEDYREALHSNLEELQWMAQLVNDMLYLAKADHGLL
TPKREPLQLAEEVDLLLEFYAPLAEDARVKLTRLGDARLNGDRSMLRRALSNLLDNALRF
TPPNGEVQVRIVDEPKGLSLSVENSGEGISEELLPRLFDRFYRADPARHEGSSEHAGLGL
AITQSIIRAHGGTIRCESGQGWTRFLMQLPKGV