Protein Info for AO353_00345 in Pseudomonas fluorescens FW300-N2E3

Annotation: NADPH-dependent 7-cyano-7-deazaguanine reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 TIGR03138: queuine synthase" amino acids 5 to 276 (272 residues), 401.4 bits, see alignment E=1.1e-124 PF14819: QueF_N" amino acids 16 to 126 (111 residues), 149.6 bits, see alignment E=4e-48 PF14489: QueF" amino acids 190 to 265 (76 residues), 76.5 bits, see alignment E=1.6e-25

Best Hits

Swiss-Prot: 96% identical to QUEF_PSEPF: NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K06879, 7-cyano-7-deazaguanine reductase [EC: 1.7.1.13] (inferred from 96% identity to pfo:Pfl01_3853)

Predicted SEED Role

"NADPH dependent preQ0 reductase (EC 1.7.1.13)" (EC 1.7.1.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VIB4 at UniProt or InterPro

Protein Sequence (276 amino acids)

>AO353_00345 NADPH-dependent 7-cyano-7-deazaguanine reductase (Pseudomonas fluorescens FW300-N2E3)
MHPAAEHSPLGKSSEYIATYTPSLLFPIPRTAKWAELGLTAETLPYKGVDFWNCFELSWL
LPSGKPVVAIGEFSIPADSPNIIESKSFKLYLNSLNQTPFADTQSLEATLVNDLSAAAGK
PVGVRIRSLNEVEAEGVVALPGVCIDDLDISVSNYEHPRPELLRCDDSRIVEESVHSHLL
KSNCPVTSQPDWGSVAVEYRGAALDHASLLAYLVSFRQHSDFHEQCVERIFLDLQRLLKP
EKLTVYARYVRRGGLDINPYRSTEEVQLPNHRLVRQ