Protein Info for ABZR87_RS22605 in Ralstonia sp. UNC404CL21Col

Annotation: NAD-glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 34 to 198 (165 residues), 173.5 bits, E=9.6e-55
• PF21073: GDH_HM1
  • amino acids 370 to 429 (60 residues), 86.4 bits, see alignment (E = 3.8e-28)
• PF21076: GDH_ACT2
  • amino acids 433 to 522 (90 residues), 122.2 bits, 4.3e-39
• PF21079: GDH_HM2
  • amino acids 528 to 570 (43 residues), 62 bits, see alignment (E = 1.6e-20)
• PF21077: GDH_ACT3
  • amino acids 582 to 659 (78 residues), 98.8 bits, 4.9e-32
• PF21078: GDH_HM3
  • amino acids 676 to 741 (66 residues), 103.5 bits, see alignment (E = 2e-33)
• PF05088: Bac_GDH_CD
  • amino acids 758 to 1260 (503 residues), 910.7 bits, E=8.6e-278
• PF21074: GDH_C
  • amino acids 1306 to 1640 (335 residues), 352.6 bits, E=9.2e-109

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-alanine degradation II (to D-lactate) (3/3 steps found)
• L-glutamate degradation I (1/1 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• methylaspartate cycle (11/19 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.2

Use Curated BLAST to search for 1.4.1.2

Protein Sequence (1644 amino acids)

>ABZR87_RS22605 NAD-glutamate dehydrogenase (Ralstonia sp. UNC404CL21Col)
MPAQHEDKVRQHMADAVALARERAPDIADLFEPFLRHYYGLADPEDVISRSVADLYGAAM
AHWQLGQKFVSGQPRVRVYNPSLEQHGWYCGHTVVEIVNDDMPFLFDSVTMEINRQGLAL
HSAFHPVYRVQRDAAGMRVAVSAGGGVQRPAALAGDMPDTPADADTETHGAARFESTIHI
EVDRFSEPERLQALHDGLMRVLGDVRAAVEDWKPMQAAAHAAIEALAVRAAQPSAGDVER
AEIAETQAFLTWMLERHFTFLGYRDYELITQDDGHYLRGIPGTGLGVLREALRDTSTPDT
TRLAPGAAKFIDAPEPIFLTKANSRATVHRPGYLDYIGIKLFDAEGRVCGQRRFLGMYTS
NVYMVPAEDIPLVRRKVADVIRRTGFLPDGHLAKTLVTILEQYPRDELFQMDAEALHDIA
LGILRLQERQRTRLFVRRDPFDRFVSCLVFVPREKFNTDLRVRIQSLLQAAYHGTAVEFT
PQLSESMLARIHITVRTQPGNVPDVDVAELEDRIVQAARRWQDDLADALLERGGEERGNR
LLRRYASAFPAGFREDYAARLAVRDIELMEPLLGANAADNVLTMQLYRPLEAPPGALRFK
IYRAGQPTSLSHSLPMLEHLGVRVNEERPYCIEPADAAPIWMHDFGMETIDGSEVDLDEA
RARFEDAFARIWSGELENDDLNRLVLQAGLTWREVRILRAYARYIRQIGSTFSNAYMESA
LNGNPSIARALVRLFLMRFDPALAEAERSRAAQTLRKQIDEALEDVPNLDEDRILRQFLG
VLEATLRTNYFQSLPDTGRGQEQGQPKPYLSFKFDPSRVPGLPEPKPMFEIWVYSPRVEG
VHLRGGKVARGGLRWSDRREDFRTEVLGLVKAQMVKNTVIVPVGSKGGFVVKQPPPASDR
DAYLAEGVACYQTFLRGLLDLTDNYVDGRVVPPRDVVRYDEDDPYLVVAADKGTATFSDY
ANAISAEYGFWLGDAFASGGSVGYDHKKMAITARGAWESVKRHFSEMGVDTQTQDFTVVG
VGDMSGDVFGNGMLLSRHVRLLAAFDHRHIFLDPSPDAATSFAERERLFNLPRSSWADYD
RALISPGGGIFPRTVKSITLTPEVRAMLDVTATEMAPNDLLHAILKAPADLLYNGGIGTY
IKASTETHAQVGDRANDGLRVNGAELRCKVVAEGGNLGCTQLGRIEYAQHGGRINTDAID
NSAGVDCSDHEVNIKILLGLVVADGEMTLKQRNTLLAEMTDEVGELVLRDNYFQTQALSL
ARTRTALWLDPEARLMRHLERSGRLNRAIEFLPADEEIDVRRASGGGLTTPERAVLMAYS
KMWLYDVLLGSDLPDQPFVADGLPAYFPRPLHTRCATSIPRHTLRREILATMHANALVNR
AGVTFVHRMAEETGAEPLAVVWASLVARAVYRLDALWEDVDALDAKVPHETQTALFTSLA
QLHERATLWFLRRRVADVPAAVEHFRAAVDALAPEIDTLQTEESALAAAQQRHSFSEAGV
PDTLARVVTGVPARVSLLDIAEVAAAGGCDARLAARVYFALDQPLGYGWLQGGILGLPTQ
THWQMLARATLLEELGQLRRRLTQSVLQGAAPGASADTLIDAWRTTRQEALARYNRVIAD
QVAAGSADLAMLSVGLKALAEVDA