Protein Info for ABZR87_RS21355 in Ralstonia sp. UNC404CL21Col

Annotation: ATP-dependent DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 548 TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 2 to 545 (544 residues), 711.5 bits, see alignment E=4e-218 PF04675: DNA_ligase_A_N" amino acids 2 to 161 (160 residues), 36.9 bits, see alignment E=6.7e-13 PF01068: DNA_ligase_A_M" amino acids 215 to 414 (200 residues), 108.3 bits, see alignment E=6.5e-35 PF04679: DNA_ligase_A_C" amino acids 435 to 530 (96 residues), 34.7 bits, see alignment E=2.9e-12

Best Hits

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 73% identity to cti:RALTA_B1594)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (548 amino acids)

>ABZR87_RS21355 ATP-dependent DNA ligase (Ralstonia sp. UNC404CL21Col)
MKAFADLYAQLDATTSSNGKLAALVAYLRTAPDADAAWAVYFLAGGKPRQLVPVRVLASF
AQTTSGLPEWLFDECYQAVGDLAETIALVLPDADHADDASLAEWMEARLLPLRGQPPEAV
LPRLAELCRGLPLHARLVLMKLITGAFRVGVSKLLVTRALAEMGGTDPKHVAQRLMGYTD
IGQAPTAERYRALLADASVDGHALDTGHPYPFFLAHPLQADVEAFDTLLGTPAEWQIEWK
WDGIRAQLVRRADSAWLWSRGEELITDRFPELSAMAEVLPPGTVLDGEIVVWQAGRVQPF
ALLQQRIGRKTLNARVLREAPAVLLAYDLLEWQGQDLREHPQSERRAQLERVIADCIHPA
LELSPLLEGDDWQHLAQQREASRELGVEGLMLKRRDARYGVGRTKDVGVWWKWKIDPFSV
DAVLIYAQRGHGRRASLYTDYTFAVWDAPPDVPERKLVPFAKAYSGLSDAEIRQVDALIR
KTTVESFGPVRSVAPTLVFELGFEGIARSPRHKSGIAVRFPRMLRWRHDKPVAEADTLQT
LEGLLPPS