Protein Info for ABZR87_RS19470 in Ralstonia sp. UNC404CL21Col

Annotation: UDP-glucose 4-epimerase GalE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 4 to 163 (160 residues), 46.5 bits, E=1.1e-15
• PF01370: Epimerase
  • amino acids 5 to 263 (259 residues), 183.7 bits, E=1.6e-57
• PF02719: Polysacc_synt_2
  • amino acids 5 to 184 (180 residues), 41.1 bits, E=5.1e-14
• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 5 to 335 (331 residues), 437.4 bits, E=1.4e-135
• PF16363: GDP_Man_Dehyd
  • amino acids 6 to 325 (320 residues), 183.8 bits, E=2.6e-57
• PF07993: NAD_binding_4
  • amino acids 7 to 164 (158 residues), 29.4 bits, E=1.8e-10
• PF01073: 3Beta_HSD
  • amino acids 7 to 161 (155 residues), 45.3 bits, E=2.4e-15
• PF13460: NAD_binding_10
  • amino acids 9 to 173 (165 residues), 30.9 bits, E=9.9e-11

Best Hits

Swiss-Prot: 62% identical to GALE_BACSU: UDP-glucose 4-epimerase (galE) from Bacillus subtilis (strain 168)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 100% identity to rpi:Rpic_4235)

MetaCyc: 62% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• colanic acid building blocks biosynthesis (7/11 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Protein Sequence (341 amino acids)

>ABZR87_RS19470 UDP-glucose 4-epimerase GalE (Ralstonia sp. UNC404CL21Col)
MTETILLTGATGYIASHTWVELLDAGYQVIGLDNLCNSSSTVLSRIEQITAKTPKFVQGD
VRDRRLLDDLFASSRISAVIHFAALKSVGESVQKPLAYYDNNMGGLVTLCAAMAHANVRQ
LVFSSSATVYGNPHTVPITESFPLSATNPYGQTKVMGEQVLRDLEISNAAWQIAYLRYFN
PVGAHHSGLIGEDPRGIPNNLMPYVAQVAVGKREKLSIYGGDWPTPDGTGVRDYIHVVDL
ARGHLSALDTLRRDGRGFTVNLGTGRGYSVLEVVEAYKRASGKPVPYQIVDRRPGDIAAC
YADPALAASLLGWRAQYGIERMCEDSWRWQSMNPDGFQTRG