Protein Info for ABZR87_RS18455 in Ralstonia sp. UNC404CL21Col

Annotation: shikimate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 TIGR00507: shikimate dehydrogenase" amino acids 4 to 272 (269 residues), 253.2 bits, see alignment E=1.2e-79 PF08501: Shikimate_dh_N" amino acids 7 to 91 (85 residues), 73.5 bits, see alignment E=2e-24 PF01488: Shikimate_DH" amino acids 121 to 197 (77 residues), 36.2 bits, see alignment E=9.3e-13 PF18317: SDH_C" amino acids 243 to 271 (29 residues), 37.8 bits, see alignment (E = 1.9e-13)

Best Hits

Swiss-Prot: 54% identical to AROE_NITMU: Shikimate dehydrogenase (NADP(+)) (aroE) from Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)

KEGG orthology group: K00014, shikimate dehydrogenase [EC: 1.1.1.25] (inferred from 83% identity to bac:BamMC406_6247)

MetaCyc: 48% identical to shikimate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Shikimate dehydrogenase. [EC: 1.1.1.25]

Predicted SEED Role

"Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 1.1.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.25

Use Curated BLAST to search for 1.1.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (277 amino acids)

>ABZR87_RS18455 shikimate dehydrogenase (Ralstonia sp. UNC404CL21Col)
MTDHYAVIGNPISHTKSPLIHGLFAQEARQDMEYTAIEGPVEPDTAFADVVRTFAAGGGK
GMNVTAPFKLKAFAMADERSERATLAGAVNAMKFEGGRILADNFDGIGLVRDIEINLGLP
MSGKRVLMLGAGGAARGALLPFLEAKPAELVIANRDFAKAQALAAQVAGRGVVVACGYAE
LEGMGRFDLVVNATSASLTGDLPPVPPSIFRPEGMAYELAYGKRLTPFLRLARNAGVRNL
ADGVGMLVEQAAEAFAWWRGVRPDTRPVIDRLTVPLD