Protein Info for ABZR87_RS17470 in Ralstonia sp. UNC404CL21Col

Annotation: CoA-acylating methylmalonate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 22 to 498 (477 residues), 745.8 bits, E=9.4e-229
• PF00171: Aldedh
  • amino acids 30 to 494 (465 residues), 483.4 bits, E=3e-149

Best Hits

Swiss-Prot: 58% identical to MMSA_ARATH: Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (ALDH6B2) from Arabidopsis thaliana

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 99% identity to rpf:Rpic12D_4088)

MetaCyc: 69% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• β-alanine degradation II (2/2 steps found)
• β-alanine degradation I (1/2 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• 2,4-dinitrotoluene degradation (1/7 steps found)
• myo-inositol degradation I (1/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (1/10 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Protein Sequence (515 amino acids)

>ABZR87_RS17470 CoA-acylating methylmalonate-semialdehyde dehydrogenase (Ralstonia sp. UNC404CL21Col)
MSAVASLASAKPAAQQDTPPTVKLLIGGEFVESQSKEWRDIVNPATQEVLARVPFATAGE
VDAAIRSAHAAFATWKNTPVGARMRIMLKFQALIREHSPRIARTLTAEQGKTLPDAEGDI
FRGLEVVEHACSVGSLQQGEFLENVAGAVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPM
AIVCGNTFVLKPSEQDPLSTMQLVELALEAGVPPGVLNVVHGGKDVVDALCTHEHVKAIS
FVGSTAVGTHVYRLGSEHGKRVQSMMGAKNHAVVLPDANREQAINALVGAAFGAAGQRCM
ATSVVVLVGAAQQWVPDLIAKAKTLKVNAGVEPGTDVGPVVSRAAKQRILGLIESGVQQG
ATLALDGRNVRVAGYEDGNFIGPTVFTDVKTDMDIYTNEIFGPVLLVLTAPTLDDAIALV
NANPFGNGVGLFTQSGAAARKFQSEIDVGQVGINIPIPVPVPYFSFTGSRGSKLGDLGPY
GKQVVQFYTQTKTVTARWFDDAVDAGGVNTTISLR