Protein Info for ABZR87_RS17215 in Ralstonia sp. UNC404CL21Col

Annotation: 1,4-alpha-glucan branching protein GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 768 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 143 to 767 (625 residues), 918.7 bits, see alignment E=8.9e-281 PF02922: CBM_48" amino acids 159 to 244 (86 residues), 81.3 bits, see alignment E=8e-27 PF00128: Alpha-amylase" amino acids 315 to 387 (73 residues), 27.4 bits, see alignment E=4e-10 PF02806: Alpha-amylase_C" amino acids 668 to 768 (101 residues), 79.4 bits, see alignment E=3.1e-26

Best Hits

Swiss-Prot: 82% identical to GLGB_RALSO: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 99% identity to rpf:Rpic12D_4050)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (768 amino acids)

>ABZR87_RS17215 1,4-alpha-glucan branching protein GlgB (Ralstonia sp. UNC404CL21Col)
MTALDFATPTKTATEPEAPIVLPPGVDQMRVDALTHGRLGDPFAVLGPHRLDTEDGTRWV
VRAFYPGARRVQAIDSRGQIITEMTPVGRTGLFHGPLRADAQDVYGHPELYRLRIIWPAG
TGHEAVQETEDPYAFGLLLGDLDLHLLNEGTHWALASCLGAQAMRIDGISGVRFAVWAPN
AQRVSVVGEFNQWDGRRHPMRLRHQAGVWELFLPSGLGVGPGARYKFELIGADGRLMVKA
DPAARQTEPPPATASVVADPAPLRWNDDAWMQTRAERQKPGAPISIYEVHAGSWLRDLED
GGRNLQWDELADRLIPYVTALGFTHIELLPVAEHPFGGSWGYQPLGLFAPSARFGAPEAF
ARFVDRCHQAGLGVIVDWVPAHFPTDAHGLAQFDGTALYEHQDPREGFHQDWNTLIYNFG
RNEVRGFLIASGLEWLERFHIDGLRVDAVASMLYRDYSRRAGEWVPNRYGGRENLEAVAF
IQQMNTVVHERCPGAITIAEESTAWPGVTAPVEFNGLGFDYKWNMGWMHDTLHYMQRDPV
YRQHHHDGMTFGLVYAFSEHFILPISHDEVVHGKGSLLGKMPGDEWQRLANLRAYLAFMW
THPGKKLLFMGSEFGQVGEWNHDASPEWHLLDDPRHRGVQRLVHDLNALYRNEPSLHARD
TAPEGFNWVIGDDRTNSVFAFLRLDGEGRPMLVVANMTPVPREGYRIGVPPADGATQWRE
VLNTDAAVYGGSDFGNGGAVDVDHVAAHGQGQSLVLRLPPLGVVVLKV