Protein Info for ABZR87_RS17210 in Ralstonia sp. UNC404CL21Col

Annotation: glycogen debranching protein GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 770 TIGR02100: glycogen debranching enzyme GlgX" amino acids 11 to 696 (686 residues), 1036.9 bits, see alignment E=0 PF02922: CBM_48" amino acids 15 to 103 (89 residues), 55 bits, see alignment E=8.3e-19 PF00128: Alpha-amylase" amino acids 199 to 280 (82 residues), 24.6 bits, see alignment E=1.5e-09

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 96% identity to rpf:Rpic12D_4049)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (770 amino acids)

>ABZR87_RS17210 glycogen debranching protein GlgX (Ralstonia sp. UNC404CL21Col)
MLRFPKTLSPGKPFPLGAQWDGLGINFAVFSANASKIELCIFDPTGRKELTRFALPECTD
EVWHGYLPGVEAGLVYGYRAYGPWEPQHGHRFNPHKLLLDPYARRLVGSLRWSDALFGYR
LNSNRGDLSFDRRDSAPAMPKAVVTDESFNWGNDIRPNTPWSRTVIYESHVRGVSMLRED
LLPPERGTFSALGDSVFIEHLQRLGITAIELLPIHAFLQDQFLVERGLRNYWGYNTLCFF
APEPSYLSAPSLHELKVAIRRLHAAGIEVLLDVVYNHTCEGSELGPTLSFRGLDNASYYR
LVPGQERYYINDTGCGNTLNLSHPRVLQMVMDSLRYWVNAFHVDGFRFDLGVTLGREGNG
FDPGSGFFDAVRQDPVLSTVKLIAEPWDVGPDGYQVGNQPPGFAEWNDRFRDGIRRFWRG
DSGQRPDLAARLTGSGDLFDRRHRRPWASVNYVASHDGFTLHDLVSYADKHNDANGEDNR
DGHNDNCSANWGAEGPTDDLAIQALRERVTRALLATVFFSNGTPMLLAGDEFGRTQDGNN
NAYCQDNALSWLDWSLLQKDPGQALARYTARLIALRKASPALRWPHYVHGNTEILPGVPD
IAWFDERGQVLSPDAWNDSEARALALRRACVETIDAEARPHVALLLINGAATDLTFTLPE
PAMDWLLAIDSAAPEEDARPHSGSTLKVAGRSVVLLLATQLATIAQIAGQPDADASGETL
GQVHDAHNAPENAPQNAPQSDMPEQPATDDERAAPTAGEAASDDNGDATR