Protein Info for ABZR87_RS16385 in Ralstonia sp. UNC404CL21Col

Annotation: PEP/pyruvate-binding domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01326: PPDK_N
  • amino acids 56 to 293 (238 residues), 186.7 bits, E=6.9e-59
• PF00391: PEP-utilizers
  • amino acids 764 to 835 (72 residues), 83.8 bits, E=5.5e-28

Best Hits

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 79% identity to rpf:Rpic12D_3918)

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• superpathway of anaerobic sucrose degradation (16/19 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (18/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.2

Use Curated BLAST to search for 2.7.9.2

Protein Sequence (842 amino acids)

>ABZR87_RS16385 PEP/pyruvate-binding domain-containing protein (Ralstonia sp. UNC404CL21Col)
MNPLVLDWPAAAAAGAAVAGGKGWQLGRMAMLGVPVPDGFVLAADASRGRSRGDAVPNAV
LAAITEALTARGWQNTPLAVRSSAPQEDSAQRSFAGIHETRLNVVGAQALADAVRAVWDS
AHAPQALAYRERFGIDTTDLAMAVVIMPMLPAVAAGIAFTGDPLTGRDDHIVINAHWGLG
EALVGGHADGDVDRLEIAPQDDTLRVVERQIGTKRRMTRGLAEGGTALADTATEDARRAV
LDDAQLITLARVARDTARALDFAQPRYDIEWVWDGQRFWIVQARPITTAARSTYPALQTQ
PTFWTRGNTREMFPTPLSPIDWSLYGHAANRMLTLAYSLAGYALLPGLERARLFGGHVYL
DVSLMQWEAFDAFGIAPNAINRMLGGTQPEIALPRTTWPQRLARGARLMRYLLRAGAARR
HAQSDLNRARKQAAQWREDAWPETTAELATCLRERFNIARRADSLFFLQGSAGGAVSGLV
DVIEKQCPGEGHALAAALMAGAEPSVTAQQSYDLMALAQTAAADIEALAWLRADKRIGAQ
WAQQLPPHSPFRQHFSAFLERYGHRAIEESYFRHPRWREQPDYLLDLVVGLIGSDARAVR
QRQQAASERAWVRLPFWLRPIVRGMLKSAVRDSNQREAARSALVAYLEALRTGLLKLADR
LVGEEGLDAPEDIFNLTLDECVAAGTGALPLRFAAQRARERRQQLNDWAAVQESDIVTEH
GDAPVASTAPIALSGETWTGTAVGAGSAQGLARLVDHAAAGTALQAGDILVAPSTDPAWT
PLFLKAGALVMETGGYLSHGAIVAREFGIPAVVNVPGIRAQLNDGETIRVDGNAGTVSRV
AG