Protein Info for ABZR87_RS15710 in Ralstonia sp. UNC404CL21Col

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 transmembrane" amino acids 501 to 521 (21 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 9 to 935 (927 residues), 1094.9 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 22 to 626 (605 residues), 470.8 bits, see alignment E=8.9e-145 PF09334: tRNA-synt_1g" amino acids 48 to 191 (144 residues), 25.9 bits, see alignment E=1.1e-09 amino acids 405 to 566 (162 residues), 20.4 bits, see alignment E=4.8e-08 PF13603: tRNA-synt_1_2" amino acids 252 to 307 (56 residues), 26.8 bits, see alignment 8.6e-10 PF08264: Anticodon_1" amino acids 677 to 831 (155 residues), 133.7 bits, see alignment E=1.2e-42 PF10458: Val_tRNA-synt_C" amino acids 889 to 953 (65 residues), 66.7 bits, see alignment 4.5e-22

Best Hits

Swiss-Prot: 83% identical to SYV_CUPNJ: Valine--tRNA ligase (valS) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 76% identity to bch:Bcen2424_1441)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (958 amino acids)

>ABZR87_RS15710 valine--tRNA ligase (Ralstonia sp. UNC404CL21Col)
MTDQNQSLAKSFEPATIEQKWSAAWEAMGVSRATLEAGKPDFCIQLPPPNVTGTLHMGHA
FNQTIMDGLTRHARMSGANTLWVPGTDHAGIATQIVVERQLDAQGVSRHDMGREKFVEKV
WEWKEQSGSTITRQVRRMGASIDWEREYFTMDPKMSRAVSDVFVRLYEQGLIYRGKRLVN
WDPVLGTAVSDLEVVSEEEEGSLWHIRYPLAEPDAVHGLTHLTVATTRPETMLGDTAVMV
HPEDERYAHLIGKFVHLPLTDRKIPVIADEYVDREFGTGVVKVTPGHDFNDYAVGQRHKL
PQLSILTLDAKIVADAPAAYAGLDRFDARKKIVEDLEAQGLLAEVKKHTLMVPRGDRTGV
VIEPMLTDQWFVAMSKPAPEGTRFPGRSIAEVALDAVQSGKIKLVPEQWVNTYNQWLNNI
QDWCISRQLWWGHQIPAWYDDAGNVYVGRTEEEAKAQAAAKGYTGALTRDDDVLDTWFSS
ALVPFSSLGWPADTPELKHFLPSTVLVTGYDIIFFWVARMVMMTLYFTGEVPFHTVYVHG
LVRDSEGKKMSKSEGNTLDPVDLIDGIALEPLLAKRTTGLRRPKDAPKVEKRTRKEFPDG
IPAFGADALRFTFASLATLGRSINFDPSRCEGYRNFCNKLWNATRFVLMNTEGQDCGMQE
CVGDCGPEGHLDFSQADRWIVSLLQRVETEVEKGFADYRFDNIANAIYKFVWDEYCDWYL
ELAKVQIQTGTPAQQRATRRTLLRVLETVLRLAHPIIPFITEELWQKVAPMAGRAKGDGT
ESLALQEYPRAALAKIDEQAEQWVQQLKALVDACRNLRGEMNISPAQRVPLYVNGDAEFL
QVAAPYVQALGKLSEVKVYTDAAAMEQDGAGAPVAIVGENKLLLKIEIDVAAEHARLSKE
IDRLRGEITKCEAKLGNESFVARAPAAVVEQEQKRVADFKATLTKLEAQIARLPVQAA