Protein Info for ABZR87_RS15265 in Ralstonia sp. UNC404CL21Col

Annotation: radical SAM family heme chaperone HemW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR00539: putative oxygen-independent coproporphyrinogen III oxidase" amino acids 42 to 388 (347 residues), 383.2 bits, see alignment E=6.1e-119 PF04055: Radical_SAM" amino acids 45 to 216 (172 residues), 92.3 bits, see alignment E=3.9e-30 PF06969: HemN_C" amino acids 346 to 408 (63 residues), 43.2 bits, see alignment E=3.4e-15

Best Hits

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 99% identity to rpf:Rpic12D_1962)

Predicted SEED Role

"Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB" in subsystem Heat shock dnaK gene cluster extended or Heme and Siroheme Biosynthesis or Queuosine-Archaeosine Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>ABZR87_RS15265 radical SAM family heme chaperone HemW (Ralstonia sp. UNC404CL21Col)
MIPIHPAGAAKTPSATAAETGDKVTSVFLQPGKISLPASPPLSLYVHVPWCVRKCPYCDF
NSHAEPEQGIPEERFLAAVRQDLEAALPMVWGRHVHTIFIGGGTPSLLSAQGLDRLLSDI
RMLLPVDADAEITMEANPGTFEAERFRSYRASGVNRLSIGIQSFNDAHLQALGRIHSAAE
ARAAIDIARAHFDNINVDLMFALPGQTLAECEADVEAALSFDTNHVSLYHLTLEPNTYFA
KYPPALPDDDAAFAMQDWIHARLAAAGYEHYEVSAYAKAGKRCKHNLNYWQFGDYLGIGP
GAHGKLSFPHRVIREMRHKHPDTYLRQAETAGGAAVMQEQREVGAADLPFEFMLNALRLT
DGFPVTLFQERTGLPLRSIERQLDVAEQRGLLTRDHVAIRPTELGQRFLNDLQALFLADD
EN