Protein Info for ABZR87_RS15225 in Ralstonia sp. UNC404CL21Col

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 806 PF13328: HD_4" amino acids 112 to 261 (150 residues), 175.6 bits, see alignment E=2.2e-55 TIGR00691: RelA/SpoT family protein" amino acids 112 to 801 (690 residues), 740.7 bits, see alignment E=7.8e-227 PF01966: HD" amino acids 131 to 230 (100 residues), 43.1 bits, see alignment E=1.7e-14 PF04607: RelA_SpoT" amino acids 321 to 431 (111 residues), 137.1 bits, see alignment E=1.1e-43 PF02824: TGS" amino acids 474 to 533 (60 residues), 73.4 bits, see alignment 4.2e-24 PF19296: RelA_AH_RIS" amino acids 546 to 696 (151 residues), 34.5 bits, see alignment E=8.3e-12 PF13291: ACT_4" amino acids 723 to 801 (79 residues), 65.3 bits, see alignment E=2.2e-21 PF01842: ACT" amino acids 729 to 792 (64 residues), 27.7 bits, see alignment E=6.4e-10

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 99% identity to rpi:Rpic_2274)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (806 amino acids)

>ABZR87_RS15225 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Ralstonia sp. UNC404CL21Col)
MPSSASGSGPASPRTGAPNLPDPRGADLVGERAALPPKSHKGASADAPAPAAKPESGDTL
FIDAVLEQTYRHLFGPTSQPAAPPRQQVVSISGLTEKLSYLKPADLKLVKEAFHFSDEAH
LGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDVIEDQGVTKSELAEKFGPKVA
ELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEK
RRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVS
RILETAQRALTDAGIPAEMTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVDTQMQCY
MTMGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTREMNQIAEAG
VAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPK
GEIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKNGDIVEVVTAPYSKPN
PAWLTFVRTGKARAAIRHFLKTAKLDEAIQLGERLLEQALRQIGIDMKAVSAQVWDRVVQ
WTGNKAREDVFADLALGRRVAAVVARRLEILLQEGGHEGDEALMAAVHTFTGDEAPAVTV
SGDEGMAMVFSSCCRPIPGDPIVGYIGKGEGLQIHVQECRVAKRLHGKDPEHWIDVMWAE
HTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGDQEATYMTFLIQVHDR
VHLADVMRALRRNPDVIRITRDRGLD