Protein Info for ABZR87_RS14910 in Ralstonia sp. UNC404CL21Col

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 724 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 12 to 693 (682 residues), 1059.9 bits, see alignment E=0 PF01138: RNase_PH" amino acids 17 to 146 (130 residues), 103 bits, see alignment E=4.4e-33 amino acids 326 to 458 (133 residues), 105.5 bits, see alignment E=7.3e-34 PF03725: RNase_PH_C" amino acids 149 to 212 (64 residues), 49.5 bits, see alignment E=8.3e-17 amino acids 463 to 531 (69 residues), 23 bits, see alignment E=1.6e-08 PF03726: PNPase" amino acids 244 to 322 (79 residues), 63.3 bits, see alignment E=6e-21 PF00013: KH_1" amino acids 558 to 615 (58 residues), 46.7 bits, see alignment 5.4e-16 PF00575: S1" amino acids 621 to 692 (72 residues), 56.5 bits, see alignment E=7.3e-19

Best Hits

Swiss-Prot: 98% identical to PNP_RALPJ: Polyribonucleotide nucleotidyltransferase (pnp) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 87% identity to cti:RALTA_A1030)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (724 amino acids)

>ABZR87_RS14910 polyribonucleotide nucleotidyltransferase (Ralstonia sp. UNC404CL21Col)
MTMFNKVVKEFQWGGHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNPKPGQDFFP
LTVDYLEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
SMNPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQYLLNPNRAQLAHSDLDLV
VAGTERAVLMVESEAQQLSEEVMLGAVVYGHEQMQIAINAIHELVRDGGKPEWDWQAAPK
NEALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYKSVATKLAEAGVEADGVEVDNILF
ELESKIVRGQILNGEPRIDGRDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGT
KSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRREVGHGRLAKRALIPVLPKD
DEFAYTIRLVSEITESNGSSSMASVCGGSLALMDAGVPLKAHVAGVAMGLILEDNKFAVL
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQ
AAMGGARTELSEHAPRMITVKINPEKIRDVIGKGGSTIQALTKETGCTIDIQEDGTITIA
STSSEGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAIVNILPGKDGLLHISEIANER
VNQVSDYVKEGQAVRVKLLSTDEKGRMRLSIKAAKAEEGDVPAAAPQAPGAGDAASQQQQ
QQQQ