Protein Info for ABZR87_RS14865 in Ralstonia sp. UNC404CL21Col

Annotation: NADH-quinone oxidoreductase subunit D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to NUOD_RALPJ: NADH-quinone oxidoreductase subunit D (nuoD) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K00333, NADH dehydrogenase I subunit D [EC: 1.6.5.3] (inferred from 100% identity to rpi:Rpic_2211)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
aerobic respiration I (cytochrome c) (3/4 steps found)
aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
NAD(P)/NADPH interconversion (3/6 steps found)
Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (417 amino acids)

>ABZR87_RS14865 NADH-quinone oxidoreductase subunit D (Ralstonia sp. UNC404CL21Col)
MADIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLHRATEKLAEQKTWIQSV
PYMDRLDYVSMMVNEHAYVMAIERLLGLEVPLRAQYIRVMFDEITRIMNHLMWIGSHALD
VGAMAVFLYAFREREDLFDMYEAVSGARMHAAYYRPGGVYRDLPDTMPQYKASKVRNEKA
LAALNETRSGSLLEFIEDFTNRFPKYVDEYETLLTDNRIWKQRLVGIGVVSPERALNKGF
SGAMLRGSGIEWDVRKKQPYEVYDRIDFDIPVGVNGDCYDRYLVRVEEMRQSNRIIRQCI
EWLRKNPGPVITDNHKVAPPSRVDMKTNMEELIHHFKLFTEGMHVPEGEAYAAVEHPKGE
FGIYAISDGANKPYRLKIRAPGFVHLAALDEMAKGHMIADAVTIIGTQDIVFGEIDR