Protein Info for ABZR87_RS14820 in Ralstonia sp. UNC404CL21Col

Annotation: NADH-quinone oxidoreductase subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 29 to 49 (21 residues), see Phobius details amino acids 72 to 101 (30 residues), see Phobius details amino acids 108 to 128 (21 residues), see Phobius details amino acids 133 to 152 (20 residues), see Phobius details amino acids 164 to 184 (21 residues), see Phobius details amino acids 205 to 224 (20 residues), see Phobius details amino acids 270 to 291 (22 residues), see Phobius details amino acids 298 to 317 (20 residues), see Phobius details amino acids 329 to 347 (19 residues), see Phobius details amino acids 368 to 390 (23 residues), see Phobius details amino acids 401 to 423 (23 residues), see Phobius details amino acids 445 to 464 (20 residues), see Phobius details TIGR01972: proton-translocating NADH-quinone oxidoreductase, chain M" amino acids 3 to 482 (480 residues), 622.7 bits, see alignment E=2.2e-191 PF01059: Oxidored_q5_N" amino acids 57 to 120 (64 residues), 24.3 bits, see alignment E=2.9e-09 PF00361: Proton_antipo_M" amino acids 130 to 411 (282 residues), 270.4 bits, see alignment E=1.9e-84

Best Hits

Swiss-Prot: 49% identical to NU4M_WHEAT: NADH-ubiquinone oxidoreductase chain 4 (ND4) from Triticum aestivum

KEGG orthology group: K00342, NADH dehydrogenase I subunit M [EC: 1.6.5.3] (inferred from 99% identity to rpf:Rpic12D_1879)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (488 amino acids)

>ABZR87_RS14820 NADH-quinone oxidoreductase subunit M (Ralstonia sp. UNC404CL21Col)
MVLSFAIWLPIFFGVLVLASGSDRNPGYVRWMSLIGSLISFVMTLPLITRFDKTTAAMQF
VEKSSWIERFHISYYLGVDGISMWFVVLTAFITVIVVIAAWEVITERVAQYMAAFLILSG
LMIGVFAALDGLLFYVFFEATLIPMYIIIGVWGGPNRVYAAFKFFLYTLLGSLLTLVALL
YLYFHSGTFEILQWHQVKLSMNEQILIFLAFFMAFAVKVPMWPVHTWLPDAHVEAPTGGS
VVLAAIMLKLGAYGFLRFSLPIAPDASHSLSAFIIAISLVAVIYIGLVALVQADMKKLVA
YSSIAHMGFVTLGFFIFNEIGVEGGIVQMISHGFISGAMFLCIGVLYDRVHSRQIADYGG
VVNTMPKFAALSVFFAMANCGLPATSGFVGEFMVILGSVKFNFWIGLLAATALIFGAAYS
LWMVKRVIFGDVTHKHVAELKDLNCREFFMLGVLAIATLYMGLYPKPFTDVMHASVVNLL
THVAQSKL