Protein Info for ABZR87_RS14255 in Ralstonia sp. UNC404CL21Col

Annotation: lipid A hydroxylase LpxO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 281 to 299 (19 residues), see Phobius details PF05118: Asp_Arg_Hydrox" amino acids 70 to 223 (154 residues), 173.7 bits, see alignment E=1.3e-55

Best Hits

KEGG orthology group: K12979, beta-hydroxylase [EC: 1.14.11.-] (inferred from 99% identity to rpf:Rpic12D_1756)

Predicted SEED Role

"Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO" in subsystem Lipid A modifications

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.11.-

Use Curated BLAST to search for 1.14.11.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (300 amino acids)

>ABZR87_RS14255 lipid A hydroxylase LpxO (Ralstonia sp. UNC404CL21Col)
MIKWIIVALYLGSILYVHFRGKVRLKFWRQMFDHSAVVAPVNCFMYAFSGVPQTPYVPVE
RFPDLEKLQAAWPQIRDEGLALINLRKIKAAEKNDDAGFNSFFKNGWKRFYLKWYEAHHP
SAEQLCPQTVALLRDLPSVKAAMFAELPPGGKLNPHRDPFAGSLRYHLGLSTPNDDRCFI
EVDGQRHSWRDGQGVVFDETYLHWAENASDKDRLILFCDIERPMKFGWAQRVNKWLGRKV
MTAASSPNDDGDQTGGINKLFRYVWVMGQYRRRFKAWNRKVYYVVKFGLIIGVAALIIWI