Protein Info for ABZR87_RS14155 in Ralstonia sp. UNC404CL21Col

Annotation: propionate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 633 transmembrane" amino acids 125 to 142 (18 residues), see Phobius details TIGR02316: propionate--CoA ligase" amino acids 6 to 630 (625 residues), 1220.5 bits, see alignment E=0 PF16177: ACAS_N" amino acids 7 to 61 (55 residues), 87.6 bits, see alignment 5.8e-29 PF00501: AMP-binding" amino acids 63 to 448 (386 residues), 262.9 bits, see alignment E=6.4e-82 PF13193: AMP-binding_C" amino acids 513 to 596 (84 residues), 80.2 bits, see alignment E=2.6e-26

Best Hits

Swiss-Prot: 64% identical to PRPE_ECOLI: Propionate--CoA ligase (prpE) from Escherichia coli (strain K12)

KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 96% identity to rpi:Rpic_2037)

MetaCyc: 64% identical to propionyl-CoA synthetase (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]

Predicted SEED Role

"Propionate--CoA ligase (EC 6.2.1.17)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 6.2.1.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (633 amino acids)

>ABZR87_RS14155 propionate--CoA ligase (Ralstonia sp. UNC404CL21Col)
MPMSAAYRALYQRSIDDPAGFWGEQAQRIDWQTPYSAVLDDSRLPFAKWFVGGRTNLCHN
AVDRHLATRGEQAALVYVSSETGVETMYTYRQLHREVNRMGASLQALGVKRGDRVLIYLP
MIPEAAFAMLACARIGAIHSVVFGGFASNSLATRIDDATPRVIVSADAGSRAGKVVEYKP
LLDAAIELAAHKPERVLLVDRKLAPMQHNARDVDYATLAAQHANADVPCEWMESSEPSYI
LYTSGTTGKPKGVQRDTGGYAVALAASIPLIFGAQPGDTMFTASDVGWVVGHSYIVYAPL
LGGLTTVMYEGTPIRPDGAVWWRIVEQYRVNVMFTAPTAIRVLKKQDPALLQRHDLSSLR
RLFLAGEPLDEPTASWIGEALQKPIVDNYWQTETGWPMLAIPQGVEPSVQKLGSPGFPVY
GYKLDILDEATGEPCAPGEKGLLAVAAPLPPGCMTTVWGDDARFLKTYWSAFLGRHIYSS
FDWGVRDEEGYVTILGRTDDVINVAGHRLGTREIEESLSSHPAIAEVAVVGVADQLKGQV
AMGFAIVRDASRIAEPAGRLALEGELMRTVEAQLGAVARPSRVFFVGALPKTRSGKLLRR
AMQAVAEGRDPGDLTTIEDPTALAQVQAALRGE