Protein Info for ABZR87_RS13985 in Ralstonia sp. UNC404CL21Col
Annotation: alpha-ketoglutarate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 52% identical to ODP1_MYCS2: Pyruvate dehydrogenase E1 component (aceE) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
KEGG orthology group: K00163, pyruvate dehydrogenase E1 component [EC: 1.2.4.1] (inferred from 82% identity to bph:Bphy_1848)Predicted SEED Role
"Pyruvate dehydrogenase E1 component (EC 1.2.4.1)" in subsystem Methionine Degradation or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.1
Use Curated BLAST to search for 1.2.4.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (899 amino acids)
>ABZR87_RS13985 alpha-ketoglutarate dehydrogenase (Ralstonia sp. UNC404CL21Col) MTDLSTGAASMRSLAHRIEDSDPGETAEWLDALEAVVQHAGTARAQFLFDRLAERAATLG VGTALARVTPYANTIPVEAQPPYPGDIDTEERLAAALRWNALAMVVRANQAYGELGGHIA SYASAADLFEVGFNHFFRADNAAHGGDLVYFQPHASPGVYARAYLEGFLDEAHLEHYRRE ITGPGLCSYPHPWLMPEFWQFPTGSMGIGPINAIYQARFMRYLHNRGLLHTTERKVWGFF GDGEMDEPESIGALSLAARERLDNIVFVINCNLQRLDGPVRGNGRIVDELEAQFTGAGWN VIKVLWGSDWDALFARDHSGALLRAFAQTVDGQFQTFSANDGAYNRERFFGQNPELAALV SHFSNEDIDRLRRGGHDVRKLHAAYARALQHTGQPTVILAKTMKGFGMGSVGQGRMTTHQ QKKLDVDQLKAFRDRFRLPLSDTDVEQLKFYKPAPNSPEMQYLHARRAALGGYLPRRRKA ATQALTVPALPSWGQFALQAEGREMSTTMAIVRMFGNLLKDDTLGPRMVPVVADEARTFG MASMFRQVGIYSPLGQQYEPEDLGSMLYYREDTGGQILEEGISEAGAISSWIAAATAYST HDLPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATAGKTTLGGEGLQHQDGASHL AASTVPNCRAWDPAFAYEVAVILDEGMRAMLERQEDTFYYLTVTNENYAQPSMPDRDLRE GILKGMYPLAPQSLPTARVQLLGSGAILGEVMAAAQMLKDDWNIDAATWSVTSFSELHRE AIAVERVARLGEKAAPSYVETVLRASRGPVVAATDYVRAVPELIRAHVPRRYVTLGTDGF GRSDSRRALRAFFEVDRASIVIAALKALADDGELDHAVVREAAARYGKHATMADAPWMR