Protein Info for ABZR87_RS13470 in Ralstonia sp. UNC404CL21Col

Annotation: pyruvate dehydrogenase (acetyl-transferring), homodimeric type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 89% identical to ODP1_CUPNH: Pyruvate dehydrogenase E1 component (pdhA) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00163, pyruvate dehydrogenase E1 component [EC: 1.2.4.1] (inferred from 80% identity to bcj:BCAL2209)

MetaCyc: 62% identical to pyruvate dehydrogenase E1 component (Escherichia coli K-12 substr. MG1655)
Pyruvate dehydrogenase (acetyl-transferring). [EC: 1.2.1.104, 1.2.4.1]; 1.2.1.104 [EC: 1.2.1.104, 1.2.4.1]

Predicted SEED Role

"Pyruvate dehydrogenase E1 component (EC 1.2.4.1)" in subsystem Methionine Degradation or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.4.1)

MetaCyc Pathways

superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
pyruvate fermentation to acetate II (3/3 steps found)
pyruvate fermentation to acetate V (2/3 steps found)
superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.1

Use Curated BLAST to search for 1.2.1.104 or 1.2.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (896 amino acids)

>ABZR87_RS13470 pyruvate dehydrogenase (acetyl-transferring), homodimeric type (Ralstonia sp. UNC404CL21Col)
MSAVPEQVLGATSASDADPQETKEWLEALQGVIGAEGPQRAAFLLDKQIEYARINGVTSP
LHAETPYINTIPVENQDRIPGNQEIEHRIRSYTRWNAMAMVLRANKDTNVGGHISSFASA
ATLYDVGYNHFWHAPSDKHGGDMVFVQGHSAPGVYSRAFLLGRLTEEQLDNFRQEVDGKG
ISSYPHPWLMPDFWQFPTVSMGLGPIMAIYQARFTKYLASRGLIQNWENRKVWAFLGDGE
TDEPESLGAIGMAGREKLDNLVFVINCNLQRLDGPVRGNGKIIQELESEFRGAGWNVIKV
IWGSRWDQLLARDTKGLLMSRMMECVDGEYQTFKSKSGAYVREHFFNTPELKAMVADWSD
DDIWALNRGGHDPHKIYAAFQSATNHKGQPTVILAKTIKGYGMGEAGEAMNIAHQQKKMP
VDAIRKLRDRFNIPVADDQLEHVPYVKFEEGSKELEYMRKARMDLGGYLPARRQKADPLQ
VPALSAFDALLKATGEGREVSTTMAFVRILNILLKDKNIGKHVVPIVPDESRTFGMEGLF
RQVGIWNQEGQKYVPQDHDQLMFYKESQTGQVLQEGINEAGAMCDWIAAATSYSTHGVAM
IPFYIYYSMFGIQRIGDLCWAAGDMRSRGFLLGGTAGRTTLNGEGLQHEDGHSHVYHAAI
PNCISYDPTFQYELAVVMQDGLRRMYVEQEDVYYYLTVMNENYEHPEMPAGVEADIIKGM
YQFKKGVENSNAPRVQLLGSGTIFREVIAAADLLKKDWGVESDLWGCPSFTELAREGNAI
ARWNLLNPTEPQRESHVEKLLKNARGPVIASTDYVRTFAEQIRAFVPRRYVVLGTDGFGR
SDTREKLRHFFEVDRYWVTVASLKALADEGVLPREKVAEALKKYNLDPNKPNPMTV