Protein Info for ABZR87_RS12790 in Ralstonia sp. UNC404CL21Col

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 10 to 413 (404 residues), 677.9 bits, see alignment E=2.7e-208 PF06689: zf-C4_ClpX" amino acids 14 to 49 (36 residues), 70 bits, see alignment 5.4e-23 PF00493: MCM" amino acids 71 to 196 (126 residues), 25.6 bits, see alignment E=2.7e-09 PF13401: AAA_22" amino acids 114 to 195 (82 residues), 29 bits, see alignment E=5.4e-10 PF07724: AAA_2" amino acids 115 to 311 (197 residues), 118.5 bits, see alignment E=1.4e-37 PF07728: AAA_5" amino acids 117 to 194 (78 residues), 27.4 bits, see alignment E=1.3e-09 PF00004: AAA" amino acids 118 to 235 (118 residues), 58.8 bits, see alignment E=3.7e-19 PF10431: ClpB_D2-small" amino acids 318 to 394 (77 residues), 43.5 bits, see alignment E=1.2e-14

Best Hits

Swiss-Prot: 100% identical to CLPX_RALPJ: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 100% identity to rpi:Rpic_1868)

MetaCyc: 75% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (424 amino acids)

>ABZR87_RS12790 ATP-dependent Clp protease ATP-binding subunit ClpX (Ralstonia sp. UNC404CL21Col)
MADKKGSTGEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIDLCNEIIRDEAAISEKEGG
LAVKSDLPTPHEIRQSLDQYVIGQEQAKKILAVAVYNHYKRLKHLGKKDDVELSKSNILL
IGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVENIIQKLLQNCNYEVDKAQ
RGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLVEGTMASVPPQGGRKHPNQDFLQ
VDTTNILFICGGAFDGLEKIIMQRSDKTGIGFGAEVQSKEERDVSEVLPQVEPEDLIKFG
LIPELIGRLPVVATLAKLDEAALMEILVEPKNAIVKQYQKLLAMEGVELEIRPSALTAIA
RKAIKRKTGARGLRSIVEHALMDVMYDLPNHKGVQKVVIDESTISDEGKPLLIYEEQPKV
AGSN