Protein Info for ABZR87_RS12785 in Ralstonia sp. UNC404CL21Col

Annotation: endopeptidase La

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 PF02190: LON_substr_bdg" amino acids 14 to 203 (190 residues), 206.3 bits, see alignment E=1.3e-64 TIGR00763: endopeptidase La" amino acids 15 to 774 (760 residues), 1011.1 bits, see alignment E=0 PF00004: AAA" amino acids 354 to 492 (139 residues), 74.4 bits, see alignment E=3e-24 PF07728: AAA_5" amino acids 354 to 486 (133 residues), 37 bits, see alignment E=8.1e-13 PF05362: Lon_C" amino acids 572 to 775 (204 residues), 326.9 bits, see alignment E=1.2e-101 PF13541: ChlI" amino acids 616 to 743 (128 residues), 32 bits, see alignment E=2.6e-11

Best Hits

Swiss-Prot: 70% identical to LON_ECOL6: Lon protease (lon) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 88% identity to bmn:BMA10247_1230)

MetaCyc: 70% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (804 amino acids)

>ABZR87_RS12785 endopeptidase La (Ralstonia sp. UNC404CL21Col)
MSGTQLLPAEQIRLPLLPLRDVVVFPHMVIPLFVGRPKSIKALEAAMEAGKSIMLVAQKT
AAKDEPTDKDLYEVGCIANILQMLKLPDGTVKVLVEGTQRANILSVTDDESHFFCEAVPV
GPEPTESAETEALRRAIVSQFDQYVKLNKKIPPEILTSLSGIDEPGRLADTIAAHLPIKL
EQKQKILEMFNVTERLESLLSQLEGEIDILQVEKRIRGRVKRQMEKSQREYYLNEQVKAI
QKELGEGEEGADLEELDKKIKAARMPKEAKKKAESEFKKLKLMSPMSAEATVVRNYIDTL
VGLPWRKKSKVNNDLSNAEQVLDQDHYGLEKVKERILEYLAVQQRVDKLKAPILCLVGPP
GVGKTSLGQSVARATNRKFVRMALGGVRDEAEIRGHRRTYIGSMPGKILQSLTKVGVRNP
LFLLDEIDKMGADFRGDPSSALLEVLDPEQNHTFQDHYIEVDFDLSDVMFVATSNSLNIP
APLLDRMEVIRLSGYTEDEKVNIARRYLLPKQIKNNGLKEGEIEVTEAALRDIIRYYTRE
AGVRSLEREVSKICRKVVKQLLLKKEAGTSVKVDSDNLDKFLGVRRFDFGLAGKEDQVGQ
VTGLAWTEVGGDLLTIEAALMPGKGNITRTGSLGDVMKESVEAARSVVRSRSARLGIKDE
MFEKRDIHIHVPEGATPKDGPSAGIAMTTALVSVLTGIPVRADVAMTGEITLRGEVLPIG
GLKEKLLAAHRGGIKLALIPEENVKDLADIPDNVKNSIEIQPVRWIDKVLELALVRKPEP
LPEEEAKPAPAKGDDGKSQEMVHH