Protein Info for ABZR87_RS11795 in Ralstonia sp. UNC404CL21Col

Annotation: NAD-dependent DNA ligase LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 813 PF01653: DNA_ligase_aden" amino acids 15 to 335 (321 residues), 374 bits, see alignment E=2.1e-115 TIGR00575: DNA ligase, NAD-dependent" amino acids 20 to 635 (616 residues), 667.9 bits, see alignment E=7.8e-205 PF03120: DNA_ligase_OB" amino acids 339 to 414 (76 residues), 114.3 bits, see alignment E=7e-37 PF03119: DNA_ligase_ZBD" amino acids 439 to 466 (28 residues), 45.3 bits, see alignment (E = 2.2e-15) PF12826: HHH_2" amino acids 559 to 621 (63 residues), 64.7 bits, see alignment 2.4e-21 PF14520: HHH_5" amino acids 561 to 611 (51 residues), 18.7 bits, see alignment 6.9e-07 PF00533: BRCT" amino acids 731 to 804 (74 residues), 61.1 bits, see alignment E=3.5e-20 PF12738: PTCB-BRCT" amino acids 740 to 795 (56 residues), 25.8 bits, see alignment 3.1e-09

Best Hits

Swiss-Prot: 96% identical to DNLJ_RALPJ: DNA ligase (ligA) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 97% identity to rpf:Rpic12D_1336)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (813 amino acids)

>ABZR87_RS11795 NAD-dependent DNA ligase LigA (Ralstonia sp. UNC404CL21Col)
MRKIAEPASGATPEARAAWLRAELNRYAHEYYVLDRPSVPDAEYDRLYRELEALESEHPE
LKTPDSPTLRVGGAVLPEFAAVRHVIPMLSIRTETDTTANGARAFDASVRRELGLDDTDP
PVEYAAELKFDGLAISLRYERGYLVQAATRGDGTTGEDVTQNIRTIRQIPLGLSPVDGHV
PDVLEVRGEVYMRRDDFEKLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLSFFA
YGLGEVVGWKGMPETHSGMLDALQAFGFPVSAERAAVKGGDGLVEFHDAIGTKRDSLPFD
IDGVVYKVNSLALQRELGFRTREPRWAVAHKYPAQEALTTVESIGVQVGRTGAITPVARL
VPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVIPEVVSVVLDRRPMDDVPGS
DLFSPQQQPKYPPFELPKTCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAGRRM
MDIEGLGERYIDNLVELDYVHGIADLYKLKLEDFLEMKRRADERDGVTPETVSAGKIATK
WAENLLEGIQASKTPPLARFLFALGIRHVGESTAKTLADWLGSLAIIRRAPAPLLLALPD
VGATVAEAIADFFAEPKNQQALDALLEAGVAPQGEHAPNAKLREKLEPAELYATLGVPKL
TATRAKQLAVAAPTLAHLASVEPEQLAGLPADVAASLLEWLASHDHRAQLTKLDALRGEL
LATMPAEAAEEGVLAGKTVVLTGTLPNLTRDEAKAMLEAAGAKVSGSVSKKTDFVVAGED
AGSKLAKAEELNVKVLDEAGMLALLKNPAGDQS