Protein Info for ABZR87_RS11300 in Ralstonia sp. UNC404CL21Col

Annotation: tRNA dihydrouridine(20/20a) synthase DusA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 344 TIGR00742: tRNA dihydrouridine synthase A" amino acids 11 to 311 (301 residues), 448.3 bits, see alignment E=8.6e-139 PF01207: Dus" amino acids 15 to 319 (305 residues), 278.3 bits, see alignment E=3.8e-87

Best Hits

Swiss-Prot: 59% identical to DUSA_ECOL6: tRNA-dihydrouridine(20/20a) synthase (dusA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 98% identity to rpf:Rpic12D_1235)

MetaCyc: 60% identical to tRNA-dihydrouridine synthase A (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase A"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (344 amino acids)

>ABZR87_RS11300 tRNA dihydrouridine(20/20a) synthase DusA (Ralstonia sp. UNC404CL21Col)
MTAERPTPNPRRLSVAPMMDWTDRHCRFFHRQISRHTWLYTEMVTTGALLHGDVPRHLDF
DEVEHPVALQLGGSEPDDLAQAARLGEQWGYKEINLNCGCPSERVQRGAFGACLMAEPKL
VAQGVKAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGTIAEAGCETFIVHARNAILKGL
SPKENREIPPLRYEVVYQLKRDFPHLEILLNGGVQTLEQIDTHLAHADGVMIGREAYHHP
HLMAAFDARYYGAGTEAPTRAAVEDAMIGYVGRWVERGGYAGAAVRHMLGLYRGVAGARG
WRRVLSDSKALGRVKTLADAHALFDEARAHLRRGEESADEAVAA