Protein Info for ABZR87_RS11250 in Ralstonia sp. UNC404CL21Col

Annotation: cystathionine beta-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01053: Cys_Met_Meta_PP
  • amino acids 12 to 390 (379 residues), 337.5 bits, E=7.6e-105
• TIGR01324: cystathionine beta-lyase
  • amino acids 12 to 393 (382 residues), 408.8 bits, E=8.8e-127

Best Hits

Swiss-Prot: 48% identical to METC_RHIL3: Putative cystathionine beta-lyase (metC) from Rhizobium leguminosarum bv. viciae (strain 3841)

KEGG orthology group: K01760, cystathionine beta-lyase [EC: 4.4.1.8] (inferred from 66% identity to bbr:BB1542)

MetaCyc: 39% identical to cysteine-S-conjugate beta-lyase monomer (Saccharomyces cerevisiae)
Cysteine-S-conjugate beta-lyase. [EC: 4.4.1.13]; 4.4.1.13 [EC: 4.4.1.13]; 4.4.1.13 [EC: 4.4.1.13]; 4.4.1.13 [EC: 4.4.1.13]; Cystathionine gamma-lyase. [EC: 4.4.1.13, 4.4.1.1, 4.4.1.28]

Predicted SEED Role

"Cystathionine beta-lyase (EC 4.4.1.8)" in subsystem Methionine Biosynthesis (EC 4.4.1.8)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of L-threonine metabolism (14/18 steps found)
• D-serine degradation (3/3 steps found)
• L-serine degradation (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-threonine degradation I (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-cysteine degradation II (2/3 steps found)
• L-methionine degradation II (2/3 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• glycine betaine degradation I (5/8 steps found)
• glycine betaine degradation III (4/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• hydrogen sulfide biosynthesis II (mammalian) (1/6 steps found)
• glutathione-mediated detoxification II (2/9 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Nitrogen metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.4.1.8

Use Curated BLAST to search for 4.4.1.1 or 4.4.1.13 or 4.4.1.28 or 4.4.1.8

Protein Sequence (395 amino acids)

>ABZR87_RS11250 cystathionine beta-lyase (Ralstonia sp. UNC404CL21Col)
MSSQKTEPRLATRLVKGGTSPAFEGGRAVNPPVVRASTVLFDSMDDMNDARRRRGTERMF
TYGARGNPTGFALEDVVAQLEGGYRTRLFPTGLAAISMVFLAFARPGDHVLIADCVYQPL
RHFVDTFLRPWGVEVTYFRADGTDVAQQFKPTTKLIYVECPGSLVYEMCDLPALAEIAHR
HGALVVADNTWGSGLQYRPLALGADVSIMAATKYLSGHSDVMMGTVTTTQACWQTLATMC
DAQGVAVSPDDAYLVLRGTRTLAARLAMHERHALEIARWLVARPEVARVFCPALPDDPGH
TLWQRDCTGTNGLVSFEFARADLQAAARFVDALTLFGIGASWGGFESLATVSNVAGTRTC
TDWSAAGPIVRLHIGLEDPQDLIDDLAQAFARVQA