Protein Info for ABZR87_RS11195 in Ralstonia sp. UNC404CL21Col

Annotation: pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 593 PF01479: S4" amino acids 215 to 257 (43 residues), 26 bits, see alignment 6.3e-10 PF00849: PseudoU_synth_2" amino acids 276 to 409 (134 residues), 64.6 bits, see alignment E=1.2e-21 TIGR00093: pseudouridine synthase" amino acids 280 to 440 (161 residues), 159.5 bits, see alignment E=2.8e-51

Best Hits

KEGG orthology group: K06178, ribosomal large subunit pseudouridine synthase B [EC: 5.4.99.12] (inferred from 95% identity to rpf:Rpic12D_1207)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70)" in subsystem Two cell division clusters relating to chromosome partitioning (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (593 amino acids)

>ABZR87_RS11195 pseudouridine synthase (Ralstonia sp. UNC404CL21Col)
MPADADAQPPRQDGGDGTEAAPRRKGLRRGLRNLVASRRQQQDGRPEGEVAEAGAADAGQ
AAQPRKSRAPRARKPKEVAGEPAVVAAEGAAPTEQAPAEGQGERRGPRGRFGKNRRRQGE
GQPAERAERSERTEGAAGKNAGGRGKQQNRNGKPQGKAGGQNQNRGGKGPKGGKGSEDVF
QYVISGAYDSEADTGGASQPPKTRELTSDDDAPKLHKILADAGLGSRRDMEDLILQGRVS
VNGLPAHIGQRILATDQVRVNGKLIQRKLPNKPPRVLLYHKPAGEIVSQSDPEGRPTVFD
ALPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELTETDR
QKLLHGIPLQDGEANFLRCVDGGGEGVNHWYHVALTEGRNREVRRMFEAVNLTVSRLIRT
RYGSFVLPRGLKRGRWQEVSAEDVRTLMGTLGMKVPTASSGGGQDRQGNRRRGPAPVLMG
PMSSGFAGEANFQSRGMGIENGNRAQPAQRRPSSPRQPDPMQTSMGYINVGGPTTLTART
RMGERPNGRGGAAGGGMRDGNRTPGNKRPGKGGGGMPNGNKAPRGGSKRGGKR