Protein Info for ABZR87_RS10915 in Ralstonia sp. UNC404CL21Col

Annotation: flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase" amino acids 23 to 412 (390 residues), 387 bits, see alignment E=4.5e-120 PF04551: GcpE" amino acids 34 to 413 (380 residues), 381.4 bits, see alignment E=2e-118

Best Hits

Swiss-Prot: 98% identical to ISPG_RALPJ: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 98% identity to rpi:Rpic_1059)

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>ABZR87_RS10915 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (Ralstonia sp. UNC404CL21Col)
MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAI
QVKELARAGSELVRITVDTPAAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPEC
AQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYNKPVRIGVNWGSLDQDLLARIMDENA
QRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC
NYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEIL
QTMGLRNFTPMVIACPGCGRTTSTVFQELAARIQSYLREQMPKWKTQYPGVEEMNVAVMG
CIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLRGERITEEFQTIVDEYVRTHY
GAQASATV