Protein Info for ABZR87_RS10345 in Ralstonia sp. UNC404CL21Col

Annotation: 3-isopropylmalate dehydratase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00170: 3-isopropylmalate dehydratase, large subunit
  • amino acids 1 to 460 (460 residues), 610.7 bits, E=7.9e-188
• PF00330: Aconitase
  • amino acids 8 to 453 (446 residues), 526.5 bits, E=3.5e-162

Best Hits

Swiss-Prot: 54% identical to LEUC_RHILO: 3-isopropylmalate dehydratase large subunit (leuC) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 76% identity to rme:Rmet_0200)

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (473 amino acids)

>ABZR87_RS10345 3-isopropylmalate dehydratase large subunit (Ralstonia sp. UNC404CL21Col)
MAKTLYQKLVESHTVAQLDAQNVLLYADLHIMNEYTSPQAFAGLAEAGRTVAVPGQHMAV
VDHIIPTHPVRVRVIQDPASALQASNLAKNCREHGIVLFDTNDALQGIEHVIAPELGMIR
PGMVVLCGDSHTTTYGAFGALGFGIGTSEVEHVLATQTLVYRLAQDMRIRVEGELPIGTT
AKDLILHIISRIGAQGARGYVVEFTGSAIAGLSMEARMTLCNMAVEAGARGALIAPDAKA
TRYVLEHARDVAHTHVSAALADWRQLFSDADAHFDVEHAFDARDVAPYVTWGTSPDQAIP
ITGRIPHPEAVEDASARALRYTGLQAGAAIEGVPVQRVFIGSCTNARIEDLRAVAGIVKG
RKVAQGVRAMVVPGSGAVRVQAEQEGIADILIASGFEWRQPGCSMCLAMNDDVLAPGERC
ASTTNRNFEGRQGRGGITHLMSPAMAAAAAITGRITDVRQLQSQPAQAEAAHV