Protein Info for ABZR87_RS10190 in Ralstonia sp. UNC404CL21Col

Annotation: translation elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 3 to 181 (179 residues), 183.7 bits, E=7.2e-58
• TIGR01393: elongation factor 4
  • amino acids 3 to 597 (595 residues), 981.7 bits, E=1.1e-299
• TIGR00231: small GTP-binding protein domain
  • amino acids 5 to 169 (165 residues), 58.6 bits, E=6.7e-20
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 44.6 bits, E=3.9e-15
• PF00679: EFG_C
  • amino acids 401 to 486 (86 residues), 81.3 bits, E=1.1e-26
• PF06421: LepA_C
  • amino acids 489 to 594 (106 residues), 166.8 bits, E=3.4e-53

Best Hits

Swiss-Prot: 99% identical to LEPA_RALPJ: Elongation factor 4 (lepA) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 99% identity to rpf:Rpic12D_0990)

MetaCyc: 70% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (599 amino acids)

>ABZR87_RS10190 translation elongation factor 4 (Ralstonia sp. UNC404CL21Col)
MDNIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMEAQVLDSMDIEKERGITIKAQTAA
LSYKAQDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
ELGVEVVPVLNKIDLPAADPENAIQEIEDVIGIDATDATRCSAKTGVGVADVLEALIAKV
PAPKGDPAAPLQALIIDSWFDNYVGVVMLVRVVNGTLRAKDKVLLMATGAQHLVEQVGVF
SPKSVPRESLSAGQVGFVIAGIKELKAAKVGDTITHVAPRKAEAPLPGFKEVKPQVFAGL
YPVEANQYEALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREF
DMDLITTAPTVVYQVQLRDGTMVQVENPAKMPADPSKIEAILEPIVTVNLYMPQDYVGAV
ITLCEQKRGSQINMSYHGRQVQLTYEIPMGEIVLDFFDRLKSVSRGYASMDYEFKEYRVS
DVVKVDILINGDKVDALSIIVHRSNSTYRGREVAAKMREIIPRQMYDVAIQAAIGANVIA
RENVKALRKNVLAKCYGGDISRKKKLLEKQKEGKKRMKQVGTVEIPQEAFLAILRVEEK