Protein Info for ABZR87_RS09930 in Ralstonia sp. UNC404CL21Col

Annotation: polyhydroxyalkanoate depolymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01849: polyhydroxyalkanoate depolymerase, intracellular
  • amino acids 2 to 412 (411 residues), 606.8 bits, E=8.5e-187
• PF06850: PHB_depo_C
  • amino acids 211 to 412 (202 residues), 329.1 bits, E=4.5e-103

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 98% identity to rpf:Rpic12D_0939)

Predicted SEED Role

"Intracellular PHB depolymerase (EC 3.1.1.-)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.-)

KEGG Metabolic Maps

• 2,4-Dichlorobenzoate degradation
• Alkaloid biosynthesis II
• Ascorbate and aldarate metabolism
• Butanoate metabolism
• Glycerophospholipid metabolism
• Retinol metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.- or 3.1.1.75

Protein Sequence (423 amino acids)

>ABZR87_RS09930 polyhydroxyalkanoate depolymerase (Ralstonia sp. UNC404CL21Col)
MLYQLHEFQRALLSPMTAWAQATAKTFTNPLSPLSLMPGAQRYAAGYELLYRLGKEYEKP
EFGIRSVKSNGRDIPIVEQTIIEKPFCRLIRFKRYADDAETIKLLKDEPVVLVCAPLSGH
HSTLLRDTVRTLLQDHKVYVTDWIDARMVPVEEGAFHLSDYINYIREFINYIGADKLHVI
SVCQPTVPVLAAISLMASDGEKTPRTMTMMGGPIDARKSPTAVNSLATNKSHSWFENNVI
YTVPANYPGHGRKVYPGFLQHAGFVAMNPDRHLSSHYDYYLNLVEGDTEDAEAHVRFYDE
YNAVLDMAAEYYLETIRDVFQEFHLANGTWVVEGKPVRPQDIKSTALFTIEGELDDISGS
GQTEAAHGLCTGIPNARKQHLTAPKCGHYGIFSGRRWREQVYPQLRDFIRRYEESTPRIK
AVA