Protein Info for ABZR87_RS09505 in Ralstonia sp. UNC404CL21Col

Annotation: ADP-glyceromanno-heptose 6-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 PF04321: RmlD_sub_bind" amino acids 1 to 173 (173 residues), 36.3 bits, see alignment E=1e-12 PF01370: Epimerase" amino acids 3 to 241 (239 residues), 152.2 bits, see alignment E=5.1e-48 TIGR02197: ADP-glyceromanno-heptose 6-epimerase" amino acids 3 to 328 (326 residues), 454.8 bits, see alignment E=7.6e-141 PF16363: GDP_Man_Dehyd" amino acids 4 to 257 (254 residues), 54.7 bits, see alignment E=3.4e-18 PF01073: 3Beta_HSD" amino acids 4 to 177 (174 residues), 39.5 bits, see alignment E=1e-13

Best Hits

Swiss-Prot: 99% identical to HLDD_RALPJ: ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) from Ralstonia pickettii (strain 12J)

KEGG orthology group: K03274, ADP-L-glycero-D-manno-heptose 6-epimerase [EC: 5.1.3.20] (inferred from 98% identity to rpf:Rpic12D_0856)

Predicted SEED Role

"ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20)" in subsystem LOS core oligosaccharide biosynthesis (EC 5.1.3.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (331 amino acids)

>ABZR87_RS09505 ADP-glyceromanno-heptose 6-epimerase (Ralstonia sp. UNC404CL21Col)
MTIIVTGAAGFIGANIVKGLNERGETDIIAVDNLTRADKFKNIVDCQISDYLDKTDFVER
FARGEFGKVRAIFHEGACSDTMETDGRYMMDNNYRYSLAVMRACLDQGVQFLYASSAATY
GASETFREAPEFERPLNVYGYSKLLFDQVVRRVMPTALSQIVGFRYFNVYGPREQHKGRM
ASVAFHNFNQFRAEGTVKLFGEYNGYPQGGQMRDFVSVEDVVKVNLFFFDNPDKSGIFNL
GTGRAQPFNDIATTVVNTLRGAEGKPALSTEELAQEGLIEYVKFPDALRGKYQCFTQADQ
SRLRAAGYTAPFLTVQEGVERYCQWLLKQPA