Protein Info for ABZR87_RS09290 in Ralstonia sp. UNC404CL21Col
Annotation: alanine--tRNA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 77% identical to SYA_BURTA: Alanine--tRNA ligase (alaS) from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 77% identity to bml:BMA10229_A0455)Predicted SEED Role
"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)
MetaCyc Pathways
- tRNA charging (20/21 steps found)
- superpathway of chorismate metabolism (44/59 steps found)
- shinorine biosynthesis (3/8 steps found)
- vanchrobactin biosynthesis (2/8 steps found)
- enterobactin biosynthesis (4/11 steps found)
- ergotamine biosynthesis (4/12 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
- bacillibactin biosynthesis (3/12 steps found)
- superpathway of ergotamine biosynthesis (5/18 steps found)
- sulfazecin biosynthesis (1/16 steps found)
- phosalacine biosynthesis (2/25 steps found)
- phosphinothricin tripeptide biosynthesis (2/25 steps found)
- corallopyronin A biosynthesis (2/30 steps found)
- colibactin biosynthesis (6/38 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.1.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (880 amino acids)
>ABZR87_RS09290 alanine--tRNA ligase (Ralstonia sp. UNC404CL21Col) MKASDIRQKFLTFFETKGHTVVRSSPLVPGNDPTLLFTNSGMVQFKDVFLGTDKRPYVRA ASVQRCLRAGGKHNDLENVGYTARHHTFFEMLGNWSFGDYFKRDALVWAWELLTQEYKLP AEKLWATVYQTDDEAYDIWTKVIGLPPERVVRIGDNKGAPYASDNFWQMAETGPCGPCSE IFYDHGPDVWGGPPGSPEADGDRYIEIWNNVFMQFDRQLDPATGEYTLTPLPAPCVDTGM GMERLAAILQHVHSNYEIDLFQALIAAAARETNTKDLANNSLRVIADHIRACSFLIVDGV IPGNEGRGYVLRRIIRRAIRHGYKLGCRAAFFHKLVDDLVAQMGDAYPELREARDRVVEV LRTEEARFFETIEHGMSILDAALGELKTSGAKMLDGELAFKLHDTYGFPLDLTQDVCREN DVIVDEAAFDAAMNRQREQARAAGKFKMAAGTLDYTGDKTTFHGYDQLVRETARVTALFV DGASVQEMHPGQTGVVVLDHTPFYAESGGQVGDQGTLKAATVWFDVADTLKVQPEVFGHH GELRTGVLKVGDAVSAEVDAVRRARTMRNHSATHLMHKALREVLGSHVQQKGSLVDAEKT RFDFSHNAPMTPLQIREVEARVNAEIFANAATSAQLMGFDDAVKNGAMALFGEKYGDEVR VLSIGSSKELCGGTHVARTGDIGLFKIVSESGVAAGIRRVEAITGDNVLHYLQTLDTRIN EAAAALRAQPSELTQRIVQVQDQVKTLEKELERLKSKLAASQGDELVSQAVDVAGIKVLA AKLDGADAKTLRETMDKLKDKLQTAAIVLGSVSDGKVALIAGVTADATAKVKAGELVNFV AQQVGGKGGGRPDMAQAGGTEPDNLPQALAGVAAWVQQKV