Protein Info for ABZR87_RS08960 in Ralstonia sp. UNC404CL21Col

Annotation: bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 2 to 187 (186 residues), 179.3 bits, E=1.3e-56
• PF08659: KR
  • amino acids 3 to 161 (159 residues), 38 bits, E=3.3e-13
• PF13561: adh_short_C2
  • amino acids 7 to 220 (214 residues), 123.9 bits, E=1.7e-39

Best Hits

Swiss-Prot: 67% identical to YDFG_SALTI: NADP-dependent 3-hydroxy acid dehydrogenase YdfG (ydfG) from Salmonella typhi

KEGG orthology group: None (inferred from 99% identity to rpf:Rpic12D_0723)

MetaCyc: 64% identical to 3-hydroxy acid dehydrogenase YdfG (Escherichia coli K-12 substr. MG1655)
RXN-16000 [EC: 1.1.1.381]; RXN-8974 [EC: 1.1.1.381, 1.1.1.298]; Serine 3-dehydrogenase. [EC: 1.1.1.381, 1.1.1.298, 1.1.1.276]

Predicted SEED Role

"3-hydroxypropionate dehydrogenase (EC 1.1.1.298)" (EC 1.1.1.298)

MetaCyc Pathways

• superpathway of L-threonine metabolism (14/18 steps found)
• L-threonine degradation III (to methylglyoxal) (2/3 steps found)
• β-alanine degradation III (1/2 steps found)
• uracil degradation III (3/5 steps found)
• aminopropanol phosphate biosynthesis II (2/4 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.276 or 1.1.1.298 or 1.1.1.381

Protein Sequence (250 amino acids)

>ABZR87_RS08960 bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG (Ralstonia sp. UNC404CL21Col)
MIVFVTGATAGFGAAIARRFVREGHRVIAAGRRQDRLDALKAELGDAVLPFVLDVLDVDA
VAALPGTLPEGWREVDVLVNNAGLALGLEPAQRADLTDWDRMIGTNCSGLVHMTRALLPA
MVERNRGHIINIGSIAGTYPYPGGNVYGATKAFVRQFSLNLRADLTGTRVRVSNVEPGLC
SGTEFSNVRFKGDDARVEKVYEGTEALTADDIAEAVYWMAALPAHFNVNAMELMPVCQSF
SATSVTRGMA