Protein Info for ABZR87_RS08885 in Ralstonia sp. UNC404CL21Col

Annotation: bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 PF00383: dCMP_cyt_deam_1" amino acids 6 to 106 (101 residues), 75.8 bits, see alignment E=3.2e-25 PF14437: MafB19-deam" amino acids 8 to 121 (114 residues), 54.1 bits, see alignment E=2.5e-18 TIGR00326: riboflavin biosynthesis protein RibD" amino acids 10 to 360 (351 residues), 362.5 bits, see alignment E=2.3e-112 TIGR00227: riboflavin-specific deaminase C-terminal domain" amino acids 152 to 363 (212 residues), 180.4 bits, see alignment E=3.6e-57 PF01872: RibD_C" amino acids 153 to 346 (194 residues), 162.6 bits, see alignment E=1.6e-51

Best Hits

Swiss-Prot: 40% identical to RIBD_BACSU: Riboflavin biosynthesis protein RibD (ribD) from Bacillus subtilis (strain 168)

KEGG orthology group: K11752, diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase [EC: 1.1.1.193 3.5.4.26] (inferred from 98% identity to rpf:Rpic12D_0708)

MetaCyc: 65% identical to diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase (Burkholderia glumae)
Diaminohydroxyphosphoribosylaminopyrimidine deaminase. [EC: 3.5.4.26]; 5-amino-6-(5-phosphoribosylamino)uracil reductase. [EC: 3.5.4.26, 1.1.1.193]

Predicted SEED Role

"Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)" in subsystem Riboflavin, FMN and FAD metabolism (EC 1.1.1.193, EC 3.5.4.26)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.193 or 3.5.4.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>ABZR87_RS08885 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD (Ralstonia sp. UNC404CL21Col)
MFSDFDHAMMQRALALAEKGLFTTTPNPRVGCVIVRDDTVIGEGYTQPAGQDHAEIQAIK
DAQSRGHDVRGATAYVTLEPCSHFGRTPPCADRLVEAGIARVVAAMEDPNPAVSGRGLQK
LRDAGVDVRCGLLEREARELNIGFVSRMMRGTPWVRVKVGASLDGRTALDNGISQWITET
EARNDGHRWRARACAILTGIGTVREDNPRLTVRAVNTPRQPRRVLIDSALDVPLDAHILT
ADGHHEPTLIFAAHPEAGRMRALSERGAEVILLPNAAGKVDLAAVLRELGRREINELHVE
AGFKLNGSLLREGLVDEVLVYLAPKVLGSGQGMFNMGPLQTLEGAAEFFFHDISRIGGDL
RILARRNPTD