Protein Info for ABZR87_RS06410 in Ralstonia sp. UNC404CL21Col

Annotation: methionine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 4 to 874 (871 residues), 1226.3 bits, E=0
• PF00809: Pterin_bind
  • amino acids 26 to 264 (239 residues), 218.1 bits, E=3.1e-68
• PF02607: B12-binding_2
  • amino acids 323 to 399 (77 residues), 83.6 bits, E=1.8e-27
• PF02310: B12-binding
  • amino acids 421 to 514 (94 residues), 60.4 bits, E=3.1e-20
• PF02965: Met_synt_B12
  • amino acids 615 to 890 (276 residues), 393.9 bits, E=8.3e-122

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 86% identity to bmu:Bmul_0387)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• folate transformations III (E. coli) (8/9 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• folate transformations II (plants) (9/11 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations I (8/13 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (905 amino acids)

>ABZR87_RS06410 methionine synthase (Ralstonia sp. UNC404CL21Col)
MTDHPMRLSGLEPFNIGESTLFVNVGERTNVTGSKAFARMILNSQFDEALAVARQQVENG
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIV
NSISLKEGEEQFAHHAKLIKRYGAAAVVMAFDEQGQADTFARKTEICKRSYDFLVNKVGF
APEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDV
VREAIHTVFLYYAIQAGMDMGIVNAGQLGVYENLDPELRERVEDVVLNRRPDATDRLLEI
ADRYKGGGTKREENLAWRQEPVEKRLAHALVHGITDYVVEDTEEVRQKIFAAGGRPIQVI
EGPLMDGMNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRQIAAAGGDVRSR
GKIVIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCNDILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDEYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
SVSVASSLLSDEGAARYVEELHADYDRIRTQHANKKATPMVSLAAARANKTKIDWSNYTP
PKPKFIGRRVFRNYDLNELAQYIDWGPFFQTWDLAGKFPDILNDEIVGESARKVFSDGKS
MLARLIAGRWLTANGVIALLPANTVNDDDIEIYTDESRSEVALTWRNIRQQSERPIIDGV
MRPNRCLADFIAPKSTGIADYIGLFAVTGGIGVQKREAAFEADNDDYSAIMLKALADRFA
EAFAECLHARVRRDLWGYAPNETLDNDALIREEYKGIRPAPGYPACPEHTVKRDMFRVLD
AQEIGMGLTEALAMTPAASVSGFYLSHPDSTYFTLGKIGQDQVDDMIARSGEDRAFVERA
LAPNL