Protein Info for ABZR87_RS06260 in Ralstonia sp. UNC404CL21Col

Annotation: acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 152.7 bits, see alignment E=1.7e-48 PF02786: CPSase_L_D2" amino acids 116 to 323 (208 residues), 255.7 bits, see alignment E=1.1e-79 PF07478: Dala_Dala_lig_C" amino acids 140 to 291 (152 residues), 37.9 bits, see alignment E=4.9e-13 PF02222: ATP-grasp" amino acids 141 to 292 (152 residues), 31.8 bits, see alignment E=3.7e-11 PF02785: Biotin_carb_C" amino acids 336 to 455 (120 residues), 111 bits, see alignment E=1.1e-35 PF21139: MCC_alpha_BT" amino acids 477 to 532 (56 residues), 28.7 bits, see alignment 4.4e-10 PF00364: Biotin_lipoyl" amino acids 604 to 667 (64 residues), 61.2 bits, see alignment E=2.4e-20

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 98% identity to rpi:Rpic_0116)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (674 amino acids)

>ABZR87_RS06260 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha (Ralstonia sp. UNC404CL21Col)
MITKLLIANRGEIACRVAATCRKLGIRTVAVYSDADANARHVAACDEAIHIGEPAARDSY
LRADRILEAARATGAQAVHPGYGFLSENEAFAKACADAGIIFVGPPASAIEAMGSKSAAK
ALMETAGVPLVPGYHGDNQDAAFLRTQADRIGYPVLIKASAGGGGKGMRVVESGDAFEAS
LASVKREASSSFGDDRVLIEKYLTRPRHIEIQVFADSLGNAVYLFERDCSVQRRHQKVLE
EAPAPGMTLERRRAMGEAAVAAARAVGYVGAGTVEFIVDEDGTFYFMEMNTRLQVEHPVT
EMITGEDLVEWQLRVASGEPLPRTQDDLHIHGHALEARIYAENPDRDFLPSIGTLKVLRT
PAAVEFTVGAQANGEPAAVRIDAGVREGDAISPYYDPMIAKLIVWGRDRDEALARMLQAL
GSFHLVGLSSNVAFLRRLVASKPFATADLDTGLIARNHDTLFPAAPDVPQDAIALAVAAL
LAREARSLRTDTRDPHSPWARGSGWRLNGAAERTLRFTHGDQSIDATLVYGRDGSRLKAG
GSEAPFTSQRHAETFTVTLGTHKATGQVHADGDAFHVFTGGAHWTLERHDPLAHAGEADD
EGGKLTAPMPGKVIAVLAAPGQTVTKGAPLVVMEAMKMEHTLTAPADGVVESVLYGVGDQ
VTEGVQLLAFKPAE